Wide distribution of plasmid mediated quinolone resistance gene, qnrS, among Salmonella spp. isolated from canal water in Thailand
Issued Date
2024-06-01
Resource Type
ISSN
13645072
eISSN
13652672
Scopus ID
2-s2.0-85197336113
Pubmed ID
38908908
Journal Title
Journal of Applied Microbiology
Volume
135
Issue
6
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Applied Microbiology Vol.135 No.6 (2024)
Suggested Citation
Toyting J., Supha N., Thongpanich Y., Thapa J., Nakajima C., Suzuki Y., Utrarachkij F. Wide distribution of plasmid mediated quinolone resistance gene, qnrS, among Salmonella spp. isolated from canal water in Thailand. Journal of Applied Microbiology Vol.135 No.6 (2024). doi:10.1093/jambio/lxae134 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/99576
Title
Wide distribution of plasmid mediated quinolone resistance gene, qnrS, among Salmonella spp. isolated from canal water in Thailand
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Abstract
Aims: This research focused on assessing the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants and antimicrobial susceptibility in Salmonella strains isolated from Thai canal water. Methods and results: From 2016 to 2020, 333 water samples were collected from six canals across Bangkok, Thailand. Salmonella spp. was isolated, PMQR genes were detected through polymerase chain reactions, and the antimicrobial susceptibility was examined using the disk diffusion method. The results indicated a 92.2% prevalence of Salmonella spp. in canal water, being serogroups B and C the most frequently detected. Overall, 35.3% of isolates harbored PMQR genes, being qnrS the most prevalent gene (97.2%, n = 137/141). Other PMQR genes, including qnrB, qnrD, oqxAB, and aac(6́)-Ib-cr, were detected. Notably, six isolates harbored multiple PMQR genes. Furthermore, 9.3% and 3.8% of the overall isolates were resistant to nalidixic acid (NAL) and ciprofloxacin (CIP), respectively. PMQR-positive isolates showed higher rates of non-susceptibility to both NAL (48.2%, n = 68/141) and CIP (92.2%, n = 130/141) compared to PMQR-negative isolates (NAL: 8.9%, n = 23/258; CIP: 11.2%, n = 30/258). Conclusions: The high prevalence of Salmonella spp., significant PMQR-positive, and reduced susceptibility isolates in canal water is of public health concern in Bangkok.