A precise gene delivery approach for human induced pluripotent stem cells using Cas9 RNP complex and recombinant AAV6 donor vectors
Issued Date
2022-07-01
Resource Type
eISSN
19326203
Scopus ID
2-s2.0-85133664765
Pubmed ID
35797389
Journal Title
PLoS ONE
Volume
17
Issue
7 July
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE Vol.17 No.7 July (2022)
Suggested Citation
Chupradit K., Thongsin N., Tayapiwatana C., Wattanapanitch M. A precise gene delivery approach for human induced pluripotent stem cells using Cas9 RNP complex and recombinant AAV6 donor vectors. PLoS ONE Vol.17 No.7 July (2022). doi:10.1371/journal.pone.0270963 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86501
Title
A precise gene delivery approach for human induced pluripotent stem cells using Cas9 RNP complex and recombinant AAV6 donor vectors
Author's Affiliation
Other Contributor(s)
Abstract
Genome editing in human induced pluripotent stem cells (hiPSCs) offers a potential tool for studying gene functions in disease models and correcting genetic mutations for cell-based therapy. Precise transgene insertion in hiPSCs represents a significant challenge. In the past decade, viral transduction has been widely used due to its high transduction efficiency; however, it can result in random transgene integration and variable transgene copy numbers. Non-viral-based strategies are generally safer but limited by their low transfection efficiency in hiPSCs. Recently, genome engineering using adeno-associated virus (AAV) vectors has emerged as a promising gene delivery approach due to AAVs’ low immunogenicity, toxicity, and ability to infect a broad range of cells. The following protocol describes the workflow for genome editing in hiPSCs using the CRISPR/Cas9 ribonucleoprotein (RNP) complex combined with the recombinant AAV serotype 6 (AAV6) donor vectors to introduce a gene of interest (GOI) fused with mCherry fluorescent reporter gene into the AAVS1 safe harbor site. This approach leads to efficient transgene insertion and is applicable to precise genome editing of hiPSCs or other types of stem cells for research purposes.