Influence of Hypoxic Condition on Cytotoxicity, Cellular Migration, and Osteogenic Differentiation Potential of Aged Periodontal Ligament Cells
Issued Date
2024-01-01
Resource Type
ISSN
13057456
eISSN
13057464
Scopus ID
2-s2.0-85194069851
Journal Title
European Journal of Dentistry
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SCOPUS
Bibliographic Citation
European Journal of Dentistry (2024)
Suggested Citation
Jaiklaew S., Tansriratanawong K. Influence of Hypoxic Condition on Cytotoxicity, Cellular Migration, and Osteogenic Differentiation Potential of Aged Periodontal Ligament Cells. European Journal of Dentistry (2024). doi:10.1055/s-0044-1786844 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98570
Title
Influence of Hypoxic Condition on Cytotoxicity, Cellular Migration, and Osteogenic Differentiation Potential of Aged Periodontal Ligament Cells
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Abstract
Objective: This study aimed to investigate and compare the influence of hypoxic conditions on cytotoxicity, cellular migration, and osteogenic differentiation of aged periodontal ligament (PDL) cells. Materials and Methods: Isolated human PDL cells from aged and young subjects were cultured under hypoxic conditions, which were treated with hydrogen peroxide (H 2 O 2) (0, 25, 50, 100, 200, and 500 μM). To assess cytotoxicity, lactate dehydrogenase release was determined by the optical density at 490 nm, and the percentage of cell death was calculated. An in vitro wound healing assay was performed over 24 to 48 hours for cellular migration determination. Osteogenic differentiation was determined by alizarin red staining and osteogenic gene expression, including the expression of runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteopontin (OPN). Results: There was a significant difference in the percentage of cell death with high hypoxic condition (200 and 500 μM) compared to low hypoxic conditions on both day 1 and 2. The highest cellular migration was depicted at 50 μM in both young and aged groups of the in vitro wound healing assay. Osteogenic gene expression of RUNX2 in the aged group was increased at 25 and 50 μM hypoxic condition at day 7, but the expression was gradually decreased after 14 days. On the contrary, the expression of ALP and OPN in the aged group was increased at day 14. Only OPN had been found to be statistically significantly different when compared with gene expression at day 7 and 14 (p < 0.05). The results showed no statistically significant differences when compared with the young and aged groups in all genes and all concentrations. Conclusion: The concentration of low hypoxic condition (25-50 μM) was proposed to promote cell viability, cellular migration, and osteogenic differentiation in aged PDL cells. We suggested that the potential of aged PDL cells for use in cell therapy for periodontal regeneration might possibly be similar to that of young PDL cells.