Green synthesis of nano-liposomes containing Bunium persicum and Trachyspermum ammi essential oils against Trichomonas vaginalis
Issued Date
2023-02-01
Resource Type
ISSN
16841182
eISSN
19959133
Scopus ID
2-s2.0-85134730728
Pubmed ID
35864068
Journal Title
Journal of Microbiology, Immunology and Infection
Volume
56
Issue
1
Start Page
150
End Page
162
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Microbiology, Immunology and Infection Vol.56 No.1 (2023) , 150-162
Suggested Citation
Siyadatpanah A., Norouzi R., Mirzaei F., Haghirosadat B.F., Nissapatorn V., Mitsuwan W., Nawaz M., Pereira M.L., Hosseini S.A., Montazeri M., Majdizadeh M., Almeida R.S., Hemati M., Wilairatana P., Coutinho H.D.M. Green synthesis of nano-liposomes containing Bunium persicum and Trachyspermum ammi essential oils against Trichomonas vaginalis. Journal of Microbiology, Immunology and Infection Vol.56 No.1 (2023) , 150-162. 162. doi:10.1016/j.jmii.2022.06.006 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84829
Title
Green synthesis of nano-liposomes containing Bunium persicum and Trachyspermum ammi essential oils against Trichomonas vaginalis
Author's Affiliation
Faculty of Tropical Medicine, Mahidol University
Toxoplasmosis Research Center MAZUMS
Infectious Diseases Research Center, Birjand University of Medical Sciences
Imam Abdulrahman Bin Faisal university
Walailak University
Birjand University of Medical Sciences
CICECO – Instituto de Materiais de Aveiro
Universidade Regional do Cariri
Shahid Sadoughi University of Medical Sciences
University of Tabriz
Nano-Biotech Foresight Company Biotechnology Campus
Toxoplasmosis Research Center MAZUMS
Infectious Diseases Research Center, Birjand University of Medical Sciences
Imam Abdulrahman Bin Faisal university
Walailak University
Birjand University of Medical Sciences
CICECO – Instituto de Materiais de Aveiro
Universidade Regional do Cariri
Shahid Sadoughi University of Medical Sciences
University of Tabriz
Nano-Biotech Foresight Company Biotechnology Campus
Other Contributor(s)
Abstract
Background: Trichomonas vaginalis, a parasitic flagellated protozoan, is one of the main non-viral sexually transmitted diseases worldwide. Treatment options for trichomoniasis are limited to nitroimidazole compounds. However, resistance to these drugs has been reported, which requires the development of new anti-Trichomonas agents that confer suitable efficacy and less toxicity. Methods: In the present work, we assessed the effectiveness of the liposomal system containing essential oils of Bunium persicum and Trachyspermum ammi against T. vaginalis in vitro. The chemical composition of B. persicum and T. ammi were analyzed using gas chromatography-mass spectrometry (GC–MS). Liposomal vesicles were prepared with phosphatidylcholine) 70%) and cholesterol)30%) using the thin-film method. The essential oils of B. persicum and T. ammi were loaded into the liposomes using the inactive loading method. Liposomal vesicles were made for two plants separately. Their physicochemical features were tested using Zeta-Sizer, AFM and SEM. The anti-Trichomonas activity was determined after 12 and 24 h of parasite cultures in TYI-S-33 medium. Results: After 12 and 24 h of administration, the IC50 of the B. persicum essential oil nano-liposomes induced 14.41 μg/mL and 45.19 μg/mL, respectively. The IC50 of T. ammi essential oil nano-liposomes induced 8.08 μg/mL and 25.81 μg/mL, respectively. Conclusions: These data suggested that nano-liposomes of the essential oils of B. persicum and T. ammi may be a promising alternative to current treatments for Trichomonas infection.