Defining physicochemical properties of urinary proteins that determine their inhibitory activities against calcium oxalate kidney stone formation
Issued Date
2024-11-01
Resource Type
ISSN
01418130
eISSN
18790003
Scopus ID
2-s2.0-85203075775
Pubmed ID
39218173
Journal Title
International Journal of Biological Macromolecules
Volume
279
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Biological Macromolecules Vol.279 (2024)
Suggested Citation
Yoodee S., Peerapen P., Thongboonkerd V. Defining physicochemical properties of urinary proteins that determine their inhibitory activities against calcium oxalate kidney stone formation. International Journal of Biological Macromolecules Vol.279 (2024). doi:10.1016/j.ijbiomac.2024.135242 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/101160
Title
Defining physicochemical properties of urinary proteins that determine their inhibitory activities against calcium oxalate kidney stone formation
Author(s)
Author's Affiliation
Corresponding Author(s)
Other Contributor(s)
Abstract
We have recently reported a set of urinary proteins that inhibited calcium oxalate (CaOx) stone development. However, physicochemical properties that determine their inhibitory activities remained unknown. Herein, human urinary proteins were chromatographically fractionated into 15 fractions and subjected to various CaOx crystal assays and identification by nanoLC-ESI-Qq-TOF MS/MS. Their physicochemical properties and crystal inhibitory activities were subjected to Pearson correlation analysis. The data showed that almost all urinary protein fractions had crystal inhibitory activities. Up to 128 proteins were identified from each fraction. Crystallization inhibitory activity correlated with percentages of Ca2+-binding proteins, stable proteins, polar amino acids, alpha helix, beta turn, and random coil, but inversely correlated with number of Ox2−-binding motifs/protein and percentage of unstable proteins. Crystal aggregation inhibitory activity correlated with percentage of stable proteins but inversely correlated with percentage of unstable proteins. Crystal adhesion inhibitory activity correlated with percentage of stable proteins and GRAVY, but inversely correlated with pI, instability index and percentages of unstable proteins and positively charged amino acids. However, there was no correlation between crystal growth inhibitory activity and any physicochemical properties. In summary, some physicochemical properties of urinary proteins can determine and may be able to predict their CaOx stone inhibitory activities.