Enhancing biohydrogen gas production in anaerobic system via comparative chemical pre-treatment on palm oil mill effluent (POME)
Issued Date
2022-11-01
Resource Type
ISSN
03014797
eISSN
10958630
Scopus ID
2-s2.0-85136100387
Pubmed ID
35988402
Journal Title
Journal of Environmental Management
Volume
321
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Environmental Management Vol.321 (2022)
Suggested Citation
Arisht S.N., Mahmod S.S., Abdul P.M., Indera Lutfi A.A., Takriff M.S., Lay C.H., Silvamany H., Sittijunda S., Jahim J.M. Enhancing biohydrogen gas production in anaerobic system via comparative chemical pre-treatment on palm oil mill effluent (POME). Journal of Environmental Management Vol.321 (2022). doi:10.1016/j.jenvman.2022.115892 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84669
Title
Enhancing biohydrogen gas production in anaerobic system via comparative chemical pre-treatment on palm oil mill effluent (POME)
Other Contributor(s)
Abstract
Biological hydrogen production using palm oil mill effluent (POME) as a carbon source through dark fermentation process has been suggested to be a promising bioenergy potential and enacts as alternative renewable energy source. Results have indicated that among various 1.5% (w/v) chemical pre-treatments (sodium hydroxide, NaOH; hydrochloric acid, HCl; sulphuric acid, H2SO4; phosphoric acid, H3PO4 and nitric acid, HNO3) on POME, using H3PO4 would generate maximum biohydrogen production of 0.193 mmol/L/h, which corresponded to a yield of 1.51 mol H2/mol TCconsumed with an initial total soluble carbohydrate concentration of 23.52 g/L. Among H3PO4 concentrations (1%–10%), the soluble carbohydrate content and the biohydrogen produced was highest and increased by 1.70-fold and 2.35-fold respectively at 2.5% (w/v), as compared to untreated POME. The batch fermentation maximum hydrogen production rate and yield of 0.208 mmol/L/h and 1.69 mol H2/mol TCconsumed were achieved at optimum pre-treatment conditions of pH 5.5 and thermophilic temperature (60 °C). This study suggests that chemical pre-treatment approaches manage to produce and improve the carbohydrate utilisation process further. Continuous fermentation in CSTR at the optimum conditions produce heightened 1.5-fold biohydrogen yield for 2.5% H3PO4 at 6 h HRT as compared to batch scale. Bacterial community via next-generation sequencing analysis at optimum HRT (6 h) revealed that Thermoanaerobacterium thermosaccharolyticum registered the highest relative frequency of 20.24%. At the class level, Clostridia, Bacilli, Bacteroidia, Thermoanaerobacteria, and Gammaproteobacteria were identified as the biohydrogen-producing bacteria in the continuous system. Insightful findings from this study suggest the substantial practical utility of dilute chemical pre-treatment in improving biohydrogen production.