Efficacy of propolis extract and eye drop solutions to suppress encystation and excystation of Acanthamoeba triangularis WU19001-T4 genotype
Issued Date
2024-01-01
Resource Type
eISSN
21678359
Scopus ID
2-s2.0-85191394322
Journal Title
PeerJ
Volume
12
Issue
4
Rights Holder(s)
SCOPUS
Bibliographic Citation
PeerJ Vol.12 No.4 (2024)
Suggested Citation
Sangkanu S., Siyadatpanah A., Norouzi R., Chuprom J., Mitsuwan W., Surinkaew S., Boonhok R., Paul A.K., Mahboob T., Sama-Ae I., Oliveira S.M.R., Jimoh T.O., De Lourdes Pereira M., Wilairatana P., Wiart C., Rahmatullah M., Sakolnapa M., Nissapatorn V. Efficacy of propolis extract and eye drop solutions to suppress encystation and excystation of Acanthamoeba triangularis WU19001-T4 genotype. PeerJ Vol.12 No.4 (2024). doi:10.7717/peerj.16937 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98221
Title
Efficacy of propolis extract and eye drop solutions to suppress encystation and excystation of Acanthamoeba triangularis WU19001-T4 genotype
Author's Affiliation
Faculty of Tropical Medicine, Mahidol University
Islamic University in Uganda
UCSI University
Hunter Medical Research Institute, Australia
Gonabad University of Medical Sciences
Chulalongkorn University
Walailak University
Universidade de Aveiro
Universiti Malaysia Sabah
University of Tasmania
CICECO – Instituto de Materiais de Aveiro
University of Tabriz
University of Development Alternative
Islamic University in Uganda
UCSI University
Hunter Medical Research Institute, Australia
Gonabad University of Medical Sciences
Chulalongkorn University
Walailak University
Universidade de Aveiro
Universiti Malaysia Sabah
University of Tasmania
CICECO – Instituto de Materiais de Aveiro
University of Tabriz
University of Development Alternative
Corresponding Author(s)
Other Contributor(s)
Abstract
Background: Propolis is a natural resinous product from a variety of plants and combines it with beeswax and salivary enzymes to create bee glue. Its potentials have been employed in the treatment of many diseases and conditions for centuries. This study, therefore, aims to determine the anti-Acanthamoeba properties of the propolis extract, eye drops coupled to some mechanisms such as inhibition of encystation and excystation. Methods: The minimum inhibitory concentrations (MICs) of the most active propolis extract were assessed in trophozoites and cysts of Acanthamoeba triangularis (A. triangularis) at 0.256 and 1 mg/mL, respectively. Results: Active eye drops inhibited trophozoites and cysts with a MIC value of 100%. At concentrations lower than their MICs values (1/2-1/16 MIC), propolis and eye drops revealed inhibition of encystation. In addition, at 1/2 MIC, both the propolis extract and eye drops showed potential inhibition of excystation. In combined sets of propolis extract and eye drops, they showed slightly increasing inhibition of encystation and excystation. Surprisingly, the MIC of chlorhexidine decreased when combined with the propolis and eye drops. SEM and TEM images displayed morphological changes in A. triangularis treated with combined sets of propolis extract and eye drops. Conclusion: From this study, a new combined eye drop solution with propolis extract was found to be helpful in reducing encystation and excystation in A. triangularis. Therefore, this combination is an alternative for the treatment of eye diseases in early stages along with reducing the entry into the cyst stage of Acanthamoeba. The results of this study revealed new anti-Acanthamoeba inhibitors with promising combined alternative therapeutic potential for the treatment of Acanthamoeba keratitis.