Detection of IgG antibodies of Brugian filariasis with crude male and female antigens of Dirofilaria immitis
Issued Date
2004
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Doungrat Riyong, Paron Dekumyoy, พารณ ดีคำย้อย, Chotechuang Panasoponkul, โชติช่วง พนโสภณกุล, Jitra Waikagul, จิตรา ไวคกุล (2004). Detection of IgG antibodies of Brugian filariasis with crude male and female antigens of Dirofilaria immitis. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/63384
Title
Detection of IgG antibodies of Brugian filariasis with crude male and female antigens of Dirofilaria immitis
Abstract
Crude antigens from male and female Dirofilaria immitis were used to detect antibody to Brugian filariasis in humans by indirect ELISA. Both antigens were tested with 42 cases of Brugian filariasis, 131 cases of 20 heterologous infections and 35 healthy controls. The results--using male and female antigens--showed sensitivity of 88.1% and 88.1%, and specificities of 64.1% and 51.8%, respectively. Cross-reaction from other helminthic infections using crude male antigen gave false-positives with 48 sera from 13 heterologous diseases at the threshold value of 0.180, while the female antigen gave 63 sera from 15 diseases, at 0.309. Serum antibodies from patients with other helminthic infections--gnathostomiasis, strongyloidiasis, hookworm infections, trichinellosis, capillariasis, angiostrongyliasis, ascariasis, trichuriasis, toxocariasis, neurocysticercosis, cystic echinococcosis, taeniasis and opisthorchiasis--resulted in false-positives with both male and female antigens. One each of sparganosis and paragonimiasis heterotremus sera cross-reacted with only crude female antigen and their OD values were close to the threshold value. Although crude male antigen showed better specificity than crude female antigen, both female and male worms are sources of antigens needed for further purification. This study provides baseline data for further serodiagnosis of Brugian filariasis using dirofilaria antigen.
Description
Joint International Tropical Medicine Meeting 2004: Ambassador Hotel, Thailand 29 November-1 December 2004: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2004. p.181.