Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells
Issued Date
2022-01-01
Resource Type
eISSN
25766422
Scopus ID
2-s2.0-85130204053
Pubmed ID
35500214
Journal Title
ACS Applied Bio Materials
Rights Holder(s)
SCOPUS
Bibliographic Citation
ACS Applied Bio Materials (2022)
Suggested Citation
Udomprasert A., Wootthichairangsan C., Duangrat R., Chaithongyot S., Zhang Y., Nixon R., Liu W., Wang R., Ponglikitmongkol M., Kangsamaksin T. Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells. ACS Applied Bio Materials (2022). doi:10.1021/acsabm.2c00114 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84220
Title
Enhanced Functional Properties of Three DNA Origami Nanostructures as Doxorubicin Carriers to Breast Cancer Cells
Author's Affiliation
Other Contributor(s)
Abstract
Previous studies have shown that chemotherapeutic efficacy could be enhanced with targeted drug delivery. Various DNA origami nanostructures have been investigated as drug carriers. Here, we compared drug delivery functionalities of three similar DNA origami nanostructures, Disc, Donut, and Sphere, that differ in structural dimension. Our results demonstrated that Donut was the most stable and exhibited the highest Dox-loading capacity. MUC1 aptamer modification in our nanostructures increased cellular uptake in MUC1-high MCF-7. Among the three nanostructures, unmodified Donut exerted the highest Dox cytotoxicity in MCF-7, and MUC1 aptamer modification did not further improve its effect, implicating that Dox delivery by Donut was efficient. However, all Dox-loaded nanostructures showed comparable cytotoxicity in MDA-MB-231 due to the innate sensitivity of this cell line to Dox. Our results successfully demonstrated that functional properties of DNA origami nanocarriers could be tuned by structural design, and three-dimensional Donut appeared to be the most efficient nanocarrier.