RNA Interference Applied to Crustacean Aquaculture
1
Issued Date
2024-11-01
Resource Type
eISSN
2218273X
Scopus ID
2-s2.0-85210602505
Journal Title
Biomolecules
Volume
14
Issue
11
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biomolecules Vol.14 No.11 (2024)
Suggested Citation
Fajardo C., De Donato M., Macedo M., Charoonnart P., Saksmerprome V., Yang L., Purton S., Mancera J.M., Costas B. RNA Interference Applied to Crustacean Aquaculture. Biomolecules Vol.14 No.11 (2024). doi:10.3390/biom14111358 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/102307
Title
RNA Interference Applied to Crustacean Aquaculture
Author's Affiliation
Faculty of Science, Mahidol University
Tecnológico de Monterrey, Querétaro
University College London
Universidad de Cádiz
CIIMAR - Interdisciplinary Centre of Marine and Environmental Research
Thailand National Center for Genetic Engineering and Biotechnology
Universidade do Porto
Center for Aquaculture Technologies
Tecnológico de Monterrey, Querétaro
University College London
Universidad de Cádiz
CIIMAR - Interdisciplinary Centre of Marine and Environmental Research
Thailand National Center for Genetic Engineering and Biotechnology
Universidade do Porto
Center for Aquaculture Technologies
Corresponding Author(s)
Other Contributor(s)
Abstract
RNA interference (RNAi) is a powerful tool that can be used to specifically knock-down gene expression using double-stranded RNA (dsRNA) effector molecules. This approach can be used in aquaculture as an investigation instrument and to improve the immune responses against viral pathogens, among other applications. Although this method was first described in shrimp in the mid-2000s, at present, no practical approach has been developed for the use of dsRNA in shrimp farms, as the limiting factor for farm-scale usage in the aquaculture sector is the lack of cost-effective and simple dsRNA synthesis and administration procedures. Despite these limitations, different RNAi-based approaches have been successfully tested at the laboratory level, with a particular focus on shrimp. The use of RNAi technology is particularly attractive for the shrimp industry because crustaceans do not have an adaptive immune system, making traditional vaccination methods unfeasible. This review summarizes recent studies and the state-of-the-art on the mechanism of action, design, use, and administration methods of dsRNA, as applied to shrimp. In addition, potential constraints that may hinder the deployment of RNAi-based methods in the crustacean aquaculture sector are considered.