Fluorine-Labeled N-Boc-l-proline as a Marker for Solid-State NMR Characterization of Biofunctionalizations on Paper Substrates
Issued Date
2022-01-01
Resource Type
ISSN
19327447
eISSN
19327455
Scopus ID
2-s2.0-85147889851
Journal Title
Journal of Physical Chemistry C
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Physical Chemistry C (2022)
Suggested Citation
Höfler M.V., Limprasart W., Rösler L., Fleckenstein M., Brodrecht M., Herr K., Schäfer J.L., Biesalski M., Breitzke H., Gutmann T. Fluorine-Labeled N-Boc-l-proline as a Marker for Solid-State NMR Characterization of Biofunctionalizations on Paper Substrates. Journal of Physical Chemistry C (2022). doi:10.1021/acs.jpcc.2c08370 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84567
Title
Fluorine-Labeled N-Boc-l-proline as a Marker for Solid-State NMR Characterization of Biofunctionalizations on Paper Substrates
Other Contributor(s)
Abstract
An efficient approach employing 4-dimethylaminopyridine and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride as the coupling reagent is presented, which enables the functionalization of cotton linter paper substrates with the 19F spin label N-boc-cis-4-fluoro-l-proline. This spin label can be easily quantified by 19F magic angle spinning (MAS) NMR experiments to determine its loading on paper substrates. During the functionalization, the spin label stays intact, as confirmed by the 1H-19F heterocorrelation (1H → 19F CP MAS FSLG HETCOR) experiments. In combination with dynamic nuclear polarization (19F MAS DNP), the N-boc-cis-4-fluoro-l-proline spin label allows us to inspect 1 μmol/g and even lower molecule loadings on paper substrates, providing a highly sensitive local probe to analyze the structure of biofunctionalizations at the nanoscale on paper substrates in the future.