Development of burkholderia pseudomallei detection based on lamptts1 gene in crude soil sample
Issued Date
2013
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Tipparat Thiangtrongjit, ทิพรัตน์ เที่ยงตรงจิตต์, Premjit Amornchai, เปรมจิตร อมรชัย, Vanaporn Wuthiekanun, วรรณพร วุฒิเอกอนันต์, Direk Limmathurotsakul, ดิเรก ลิ้มมธุรสกุล, Day, Nicholas PJ, Piengchan Sonthayanon, เพียงจันทร์ สนธยานนท์ (2013). Development of burkholderia pseudomallei detection based on lamptts1 gene in crude soil sample. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/63356
Title
Development of burkholderia pseudomallei detection based on lamptts1 gene in crude soil sample
Other Contributor(s)
Abstract
Melioidosis is the third most common cause of death from infectious diseases, after HIV/AIDS
and tuberculosis in Northeastern Thailand. It caused by gram negative bacterium Burkholderia
pseudomallei which their natural habitats are soil and surface water. Detection of B. pseudomallei in
soil is important for defining geographical areas and indicating a risk of melioidosis in human and animals.
Bacterial culture is conventional method for B. pseudomallei detection from soil sample. Although, it is simple
but laborious and time consuming for large number of soil sample such as soil surveys or screening. Loopmediated
isothermal amplification (LAMP) targeting the TSS1 gene was developed to detect B. pseudomallei
in soil sample collected from Northeastern Thailand. 10 g. of soil was suspended in 10 ml TBSS-C50 broth at
40 °C for 48 hr. 250 μl of soil suspension was boiled at 100 °C for 10 min and then used for direct detection of
B. pseudomallei without prior extraction. In addition, we performed methods to eliminate inhibitors in soil by
adding 10 mg/ml bovine serum albumin (BSA) into the reaction. The results demonstrated that the detection
of LAMP product was clearly seen when adding BSA with boiling methods.
Description
Joint International Tropical Medicine Meeting 2013: Towards global health: an Asian paradigm of Tropical Medicine 11-13 December 2013 Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2013. p.161.