Green Flow Injection Spectrophotometry with Betel Nut (Areca catechu L.) Natural Reagent for Indirect Determination of Ascorbic Acid
Issued Date
2022-10-01
Resource Type
ISSN
10619348
eISSN
16083199
Scopus ID
2-s2.0-85139481954
Journal Title
Journal of Analytical Chemistry
Volume
77
Issue
10
Start Page
1274
End Page
1281
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Analytical Chemistry Vol.77 No.10 (2022) , 1274-1281
Suggested Citation
Supharoek S.a., Weerasuk B., Grudpan K., Ponhong K. Green Flow Injection Spectrophotometry with Betel Nut (Areca catechu L.) Natural Reagent for Indirect Determination of Ascorbic Acid. Journal of Analytical Chemistry Vol.77 No.10 (2022) , 1274-1281. 1281. doi:10.1134/S1061934822100148 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84154
Title
Green Flow Injection Spectrophotometry with Betel Nut (Areca catechu L.) Natural Reagent for Indirect Determination of Ascorbic Acid
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Abstract: A green flow injection spectrophotometric technique for the determination of ascorbic acid was described based on the redox and complexation reactions. Iron(III) was reduced to iron(II) by ascorbic acid with subsequent complexation reaction of remaining iron(III) with a natural reagent extracted from Areca catechu L. at pH 5.0 yielding a dark-blue color. Absorbance decreased with increasing ascorbic acid concentration. A linear range of 1–10 mg/L for ascorbic acid was obtained with r2 of 0.9986. Limit of detection and limit of quantification were 0.3 and 0.9 mg/L, respectively. Relative standard deviation was less than 5.0%. This method was utilized to quantify ascorbic acid in pharmaceuticals and soft drinks with a sampling rate of 40 h−1. Ascorbic acid contents of 96–100% in pharmaceutical samples were obtained, while recoveries for spiked ascorbic acid standard at 3 mg/L in soft drinks were 92–113%.