Low monocyte to neutrophil ratio in peripheral blood associated with disease complication in primary plasmodium falciparum infection
Issued Date
2014
Resource Type
Language
eng
Rights
Mahidol University
Suggested Citation
Piyatida Tangteerawatana, Srivicha Krudsood, ศรีวิชา ครุฑสูตร, Naowarat Kanchanakhan, Marita Troye-Blomberg, Srisin Khusmith, ศรีสิน คูสมิทธิ์ (2014). Low monocyte to neutrophil ratio in peripheral blood associated with disease complication in primary plasmodium falciparum infection. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/63159
Title
Low monocyte to neutrophil ratio in peripheral blood associated with disease complication in primary plasmodium falciparum infection
Abstract
Immunity to malaria can be acquired but only after repeat exposures to
polymorphic Plasmodium. However, the development of clinical outcomes
during P. falciparum infection is not clearly understood. This study elucidated
whether monocytes, neutrophils and pro/anti-inflammatory cytokines were associated
with clinical outcomes in single infection and prior repeated malaria infections. Two
hundred and seventy-nine patients with complicated and uncomplicated malaria
were investigated. Peripheral blood IFN-γ, TNF-αand IL-10 levels were measured by
ELISA, and monocytes and neutrophils by an automated cell counter. On admission,
in patients with uncomplicated malaria prior repeated infections, absolute neutrophil
counts were positively and monocyte to neutrophil ratio negatively correlated
significantly with parasitemia (r = 0.358, p = 0.000; r = -0.356, p = 0.000, respectively),
while those with single infection absolute monocyte counts and monocyte to neutrophil
ratio were significantly correlated negatively with IFN-γ (r = -0.381, p = 0.001; r =
-0.393, p = 0.000), and positively with TNF-α levels (r = 0.310, p = 0 .007; r = 0.227, p
= 0.017). In sharp contrast, in complicated malaria with single infection extremely high
IFN-γ and IL-10 levels but significantly low percent monocyte counts and monocyte to
neutrophil ratio were seen. After 7 days of treatment, absolute monocyte counts and
monocyte to neutrophil ratio were significantly increased, while absolute neutrophil
counts significantly decreased (p = 0.000, 0.000, and 0.001), similarly after 28 days
of treatment (p = 0.008, 0.000 and 0.000). These results suggest different functions
of monocytes, neutrophils and pro/anti-inflammatory cytokines in complicated and
uncomplicated malaria with single P. falciparum infection or prior repeated infections
in the context of disease severity. Low monocyte to neutrophil ratio may be regarded
as a risk factor in developing complication in primary malaria infection.
Description
Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 231.