WSSV early protein WSSV004 enhances viral replication by suppressing LDH activity
Issued Date
2024-06-01
Resource Type
ISSN
01418130
eISSN
18790003
Scopus ID
2-s2.0-85194961375
Pubmed ID
38763244
Journal Title
International Journal of Biological Macromolecules
Volume
271
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Biological Macromolecules Vol.271 (2024)
Suggested Citation
Ng Y.S., Chen C.Y., Cheng S.W., Tan Y.K., Lin S.S., Senapin S., Sangsuriya P., Wang H.C. WSSV early protein WSSV004 enhances viral replication by suppressing LDH activity. International Journal of Biological Macromolecules Vol.271 (2024). doi:10.1016/j.ijbiomac.2024.132482 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98671
Title
WSSV early protein WSSV004 enhances viral replication by suppressing LDH activity
Corresponding Author(s)
Other Contributor(s)
Abstract
White spot syndrome virus (WSSV) is known to upregulate glycolysis to supply biomolecules and energy for the virus's replication. At the viral genome replication stage, lactate dehydrogenase (LDH), a glycolytic enzyme, shows increased activity without any increase in expression. In the present study, yeast 2-hybrid screening was used to identify WSSV proteins that interacted with LvLDH isoform 1 and 2, and these included the WSSV early protein WSSV004. The interaction between WSSV004 and LvLDH1/2 was confirmed by co-immunoprecipitation. Immunofluorescence showed that WSSV004 co-localized with LvLDH1/2 in the cytoplasm. dsRNA silencing experiments showed that WSSV004 was crucial for WSSV replication. However, although WSSV004 silencing led to the suppression of total LvLDH gene expression during the viral late stage, there was nevertheless a significant increase in LvLDH activity at this time. We also used affinity purification-mass spectrometry to identify cellular proteins that interact with WSSV004, and found a total of 108 host proteins and 3 WSSV proteins with which it potentially interacts. Bioinformatics analysis revealed that WSSV004 and its interacting proteins might be responsible for various biological pathways during infection, including vesicular transport machinery and RNA-related functions. Collectively, our study suggests that WSSV004 serves as a multifunctional modulator to facilitate WSSV replication.