Metabarcoding in gut protozoology
Issued Date
2024-01-01
Resource Type
ISSN
14714922
eISSN
14715007
Scopus ID
2-s2.0-85208561668
Journal Title
Trends in Parasitology
Rights Holder(s)
SCOPUS
Bibliographic Citation
Trends in Parasitology (2024)
Suggested Citation
Stensvold C.R. Metabarcoding in gut protozoology. Trends in Parasitology (2024). doi:10.1016/j.pt.2024.10.015 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/102101
Title
Metabarcoding in gut protozoology
Author(s)
Author's Affiliation
Corresponding Author(s)
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Abstract
Next-generation sequencing (NGS) methods include whole-genome sequencing, metagenomic analysis, and amplicon-based NGS, all of which are gaining territory in parasitology. A modality of particular interest within the field of gut protozoology is exhaustive metabarcoding of ribosomal genes in a complex matrix such as faeces, by which method, amplicon-based NGS enables the detection and differentiation of both eukaryotic and prokaryotic organisms, circumventing Sanger sequencing-based limitations and representing a one-fits-most approach. Apart from being a tool to break the code of intracellular genetic variation and tell mixed species infections apart, metabarcoding can produce data that can serve to augment our understanding of the interplay between the organisms within the gut.