A novel allele of B(A) blood group detected in a donor and a patient during a retrospective review of ABO group anomalies in a tertiary hospital
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Issued Date
2025-06-01
Resource Type
ISSN
14730502
eISSN
18781683
Scopus ID
2-s2.0-86000660327
Journal Title
Transfusion and Apheresis Science
Volume
64
Issue
3
Rights Holder(s)
SCOPUS
Bibliographic Citation
Transfusion and Apheresis Science Vol.64 No.3 (2025)
Suggested Citation
Suksard K., Millard G.M., Teravichitchainan U., Permpikul P., Kittivorapart J. A novel allele of B(A) blood group detected in a donor and a patient during a retrospective review of ABO group anomalies in a tertiary hospital. Transfusion and Apheresis Science Vol.64 No.3 (2025). doi:10.1016/j.transci.2025.104108 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/106756
Title
A novel allele of B(A) blood group detected in a donor and a patient during a retrospective review of ABO group anomalies in a tertiary hospital
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Abstract
Background: ABO discrepancies, inconclusive results between forward and reverse typing, are one of the significant challenges encountered in transfusion medicine. Their frequency and etiologies can vary among ethnicities. This study aimed to characterize ABO discrepancies in a Thai population. Methods: We conducted a retrospective review of 285,450 donor and 258,780 patient samples for ABO discrepancies, which were categorized into five groups, as described below. The serological ABO grouping was performed using an automated system, and further serological techniques were used in the discrepancy cases. Additionally, sequencing was used to examine the genetic background of the B(A) phenotype detected during the retrospective review. Results: ABO discrepancies were identified in 396 patients (0.15 %) and 74 blood donors (0.03 %). Among the patients, the most frequent cause was ABO mismatch stem cell transplantation (198; 50 %). The remaining 198 discrepancy cases (198/258,780; 0.08 %) were categorized into five groups: weak/missing red cell reactivity, extra red cell reactivity, mixed-field, weak/missing serum reactivity, and extra serum reactivity, accounting for 17.17 %, 0.51 %, 29.29 %, 28.79 %, and 24.24 %, respectively. For the blood donors, the percentages were 48.65 %, 2.70 %, 2.70 %, 37.84 %, and 8.11 %, respectively. We also identified the B(A) phenotype in one patient and two blood donors. The sequencing study identified allele variants of c.467 C>T, c.796 C>A, c.803 G>C, and c.930 G>A in exon 7, which was a novel allele. Conclusion: ABO discrepancies were distinct between donors and patients even in the same ethnicity. This finding highlighted the influence of the patient's conditions and therapy on the anomalous ABO typing. Additionally, the B(A) individuals identified in this study carried identical genetic alterations that differed from all antecedent alleles of the B(A) phenotype.