Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering
Issued Date
2023-02-01
Resource Type
eISSN
20734360
Scopus ID
2-s2.0-85149040758
Journal Title
Polymers
Volume
15
Issue
4
Rights Holder(s)
SCOPUS
Bibliographic Citation
Polymers Vol.15 No.4 (2023)
Suggested Citation
Phuegyod S., Pramual S., Wattanavichean N., Assawajaruwan S., Amornsakchai T., Sukho P., Svasti J., Surarit R., Niamsiri N. Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering. Polymers Vol.15 No.4 (2023). doi:10.3390/polym15040855 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81749
Title
Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering
Other Contributor(s)
Abstract
In this study, we fabricated three dimensional (3D) porous scaffolds of poly(hydroxybutyrate-co-hydroxyvalerate) with 50% HV content. P(HB-50HV) was biosynthesized from bacteria Cupriavidus necator H16 and the in vitro proliferation of dental cells for tissue engineering application was evaluated. Comparisons were made with scaffolds prepared by poly(hydroxybutyrate) (PHB), poly(hydroxybutyrate-co-12%hydroxyvalerate) (P(HB-12HV)), and polycaprolactone (PCL). The water contact angle results indicated a hydrophobic character for all polymeric films. All fabricated scaffolds exhibited a high porosity of 90% with a sponge-like appearance. The P(HB-50HV) scaffolds were distinctively different in compressive modulus and was the material with the lowest stiffness among all scaffolds tested between the dry and wet conditions. The human gingival fibroblasts (HGFs) and periodontal ligament stem cells (PDLSCs) cultured onto the P(HB-50HV) scaffold adhered to the scaffold and exhibited the highest proliferation with a healthy morphology, demonstrating excellent cell compatibility with P(HB-50HV) scaffolds. These results indicate that the P(HB-50HV) scaffold could be applied as a biomaterial for periodontal tissue engineering and stem cell applications.