Effects of a specific synbiotic blend on fecal short-chain fatty acids and gut inflammation in cow's milk-allergic children receiving amino acid–based formula during early life: results of a randomized controlled trial (PRESTO study)
Issued Date
2025-01-01
Resource Type
eISSN
26736101
Scopus ID
2-s2.0-105025097257
Journal Title
Frontiers in Allergy
Volume
6
Rights Holder(s)
SCOPUS
Bibliographic Citation
Frontiers in Allergy Vol.6 (2025)
Suggested Citation
Chatchatee P., Breedveld A.C., Eussen S.R.B.M., Nowak-Wegrzyn A., Lange L., Benjaponpitak S., Chong K.W., Sangsupawanich P., Wopereis H., Oude Nijhuis M.M., Langford J.E., Kostadinova A.I., Trendelenburg V., Pesek R., Davis C.M., Muraro A., Erlewyn-Lajeunesse M., Fox A.T., Michaelis L.J., Beyer K. Effects of a specific synbiotic blend on fecal short-chain fatty acids and gut inflammation in cow's milk-allergic children receiving amino acid–based formula during early life: results of a randomized controlled trial (PRESTO study). Frontiers in Allergy Vol.6 (2025). doi:10.3389/falgy.2025.1667162 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/113698
Title
Effects of a specific synbiotic blend on fecal short-chain fatty acids and gut inflammation in cow's milk-allergic children receiving amino acid–based formula during early life: results of a randomized controlled trial (PRESTO study)
Author's Affiliation
Baylor College of Medicine
Charité – Universitätsmedizin Berlin
NYU Grossman School of Medicine
Guy's and St Thomas' NHS Foundation Trust
University Hospital Southampton NHS Foundation Trust
Azienda Ospedale Università Padova
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Faculty of Medicine, Chulalongkorn University
Arkansas Children's Hospital
Faculty of Medicine, Prince of Songkla University
Great North Children's Hospital
St. Marien Hospital, Bonn
K.K. Women's and Children's Hospital
Charité – Universitätsmedizin Berlin
NYU Grossman School of Medicine
Guy's and St Thomas' NHS Foundation Trust
University Hospital Southampton NHS Foundation Trust
Azienda Ospedale Università Padova
Faculty of Medicine Ramathibodi Hospital, Mahidol University
Faculty of Medicine, Chulalongkorn University
Arkansas Children's Hospital
Faculty of Medicine, Prince of Songkla University
Great North Children's Hospital
St. Marien Hospital, Bonn
K.K. Women's and Children's Hospital
Corresponding Author(s)
Other Contributor(s)
Abstract
Consumption of an amino acid-based formula (AAF) with added synbiotics [short-chain oligofructose and long-chain inulin (scFOS/lcFOS, 9:1 ratio) and Bifidobacterium breve M-16V] (AAF-S) beneficially impacts the gut microbiome of infants with cow's milk allergy (CMA). We assessed the effect of consuming AAF with or without synbiotics by children with CMA for 12 months on their fecal (branched) short-chain fatty acids (SCFA/BCFA) concentrations, and on gut barrier and inflammation markers (Netherlands Trial Register NTR3725). Feces and saliva were collected from 161 children (≤13 months) with IgE-mediated CMA at baseline, 6 and 12 months after enrollment, and at 24 and 36 months follow-up. Fecal SCFA and BCFA were analyzed by gas chromatography, and gut barrier and inflammation markers were measured in saliva/feces by ELISA or ImmunoCAP. At 6 months, children receiving AAF-S had significantly lower fecal propionate, valerate and BCFA concentrations compared to children consuming AAF. The percentage of propionate from the total 6 SCFA/BCFA (acetate + butyrate + propionate + valerate + isobutyrate + isovalerate) was significantly lower, while the percentage of acetate from the total 6 SCFA/BCFA was significantly higher in the AAF-S group. There were no significant differences between groups in fecal concentrations of butyrate at 6 months, nor in SCFA or BCFA at baseline and after 12, 24 or 36 months. Intestinal inflammation and barrier markers did not differ between groups. Addition of synbiotics to AAF brings concentrations of key fecal microbial metabolites more in line with patterns observed in healthy breastfed infants. The effects on SCFA and BCFA concentrations were transient and only seen at 6 months.