Two approaches for mercury determination in environmental and food monitoring via catalytic fluorometry of Au-Hg amalgamation using a laser-printed paper sensor and microcentrifuge tube test kit
2
Issued Date
2025-09-01
Resource Type
ISSN
00263672
eISSN
14365073
Scopus ID
2-s2.0-105012770950
Journal Title
Microchimica Acta
Volume
192
Issue
9
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microchimica Acta Vol.192 No.9 (2025)
Suggested Citation
Malahom N., Boonthod S., Veerasup N., Pajarean C., Prakobkij A., Anutrasakda W., Citterio D., Amatatongchai M., Nacapricha D., Jarujamrus P. Two approaches for mercury determination in environmental and food monitoring via catalytic fluorometry of Au-Hg amalgamation using a laser-printed paper sensor and microcentrifuge tube test kit. Microchimica Acta Vol.192 No.9 (2025). doi:10.1007/s00604-025-07412-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/111646
Title
Two approaches for mercury determination in environmental and food monitoring via catalytic fluorometry of Au-Hg amalgamation using a laser-printed paper sensor and microcentrifuge tube test kit
Corresponding Author(s)
Other Contributor(s)
Abstract
Two selective methods were developed for detecting mercury ions (Hg<sup>2</sup>⁺) in food and water samples using catalytic fluorometry based on Au-Hg amalgamation with enhanced peroxidase-like activity. These approaches include (i) a laser-printed microfluidic paper-based analytical device (LP-µPAD) and (ii) a paper-based microcentrifuge tube test kit. The LP-µPAD was fabricated via commercial laser printing and integrated gold nanoparticles (AuNPs) with o-phenylenediamine (OPD) and hydrogen peroxide (H₂O₂). The test kit enabled Hg<sup>2</sup>⁺ detection in food by reducing Hg<sup>2</sup>⁺ to Hg⁰ with stannous chloride, facilitating Au-Hg amalgam formation in the detection zone. Both approaches utilized the Au-Hg amalgam to catalyze H₂O₂-mediated oxidation of OPD, generating fluorescent 2,3-diaminophenazine (DAP), further enhancing fluorescence intensity in proportion to Hg<sup>2</sup>⁺ concentrations. The LP-µPAD exhibited a detection range of 3.0–20.0 µg L⁻<sup>1</sup> with a limit of detection (LOD) of 1.65 µg L⁻<sup>1</sup>, whereas the test kit provided a detection range of 0.1–1.0 mg L⁻<sup>1</sup> with an LOD of 0.08 mg L⁻<sup>1</sup>. Both sensors showed high selectivity for Hg<sup>2</sup>⁺ over other ions and performed well in real sample analyses, aligning closely with results from conventional methods.