Effect of microencapsulation using β-cyclodextrin and β-glucan as coating agents on physicochemical properties and phytocannabinoids retention of cannabis flower oil extract
2
Issued Date
2025-03-01
Resource Type
eISSN
23523646
Scopus ID
2-s2.0-85212323923
Journal Title
NFS Journal
Volume
38
Rights Holder(s)
SCOPUS
Bibliographic Citation
NFS Journal Vol.38 (2025)
Suggested Citation
Saiyasombat W., Yimpetch C., Chathiran W., Chimasangkanan J., Srichamnong W. Effect of microencapsulation using β-cyclodextrin and β-glucan as coating agents on physicochemical properties and phytocannabinoids retention of cannabis flower oil extract. NFS Journal Vol.38 (2025). doi:10.1016/j.nfs.2024.100209 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/102897
Title
Effect of microencapsulation using β-cyclodextrin and β-glucan as coating agents on physicochemical properties and phytocannabinoids retention of cannabis flower oil extract
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Abstract
Phytocannabinoids possess various biological activities; however, their limited aqueous solubility and poor stability present problems for effective utilization. Encapsulation is a highly effective method to improve the stability and solubility of cannabis extracts. The potential of β-glucan as a coating material for cannabis extract has not been extensively studied. This study aimed to investigate encapsulation of cannabis extracts by freeze-drying using β-glucan or β-cyclodextrin as coating agents. The extract was mixed with coating agent, with core-to-coating ratio of 1:1 and 1:2 (w/w). Tween 20 or 40 % ethanol were employed for preparing emulsion. Characterization was performed using contact angle, SEM, FT-IR, NMR and TGA. Phytocannabinoids and antioxidant properties were analyzed using LC-MS/MS and three antioxidant assays (DPPH, FRAP and ORAC). Results showed that different encapsulation conditions affect the phytocannabinoids contents and their morphology. The microencapsulation with Tween 20 at core-to-coating ratio of 1:1 can retain CBD and THC at 0.19 μg/g oil and 0.04–0.07 μg/g oil. SEM image showed granular structure in β-glucan encapsulated products. FT-IR, NMR and TGA confirmed microencapsulation formation. These microcapsules demonstrated high thermal stability with encapsulation efficiency ranging from 13.38 % to 64.80 %. All microcapsules exhibited various antioxidant activity. This study demonstrates the potential for developing water-soluble cannabinoids for food and pharmaceutical applications.