In vitro bactericidal efficacy of a new triple antibiotic paste formulation against Enterococcus faecalis biofilm
Issued Date
2022-01-01
Resource Type
ISSN
13291947
eISSN
17474477
Scopus ID
2-s2.0-85137485821
Journal Title
Australian Endodontic Journal
Rights Holder(s)
SCOPUS
Bibliographic Citation
Australian Endodontic Journal (2022)
Suggested Citation
Smittiset B., Banomyong D., Ruangsawasdi N., Kaewprag J. In vitro bactericidal efficacy of a new triple antibiotic paste formulation against Enterococcus faecalis biofilm. Australian Endodontic Journal (2022). doi:10.1111/aej.12683 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/87522
Title
In vitro bactericidal efficacy of a new triple antibiotic paste formulation against Enterococcus faecalis biofilm
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
The aim of this study was to evaluate the bactericidal efficacy on Enterococcus faecalis biofilm of a novel, low-concentration triple-antibiotic paste (TAP-L2) in propylene glycol/carboxymethylcellulose vehicle (VEH-2), a ready-to-use prototype, compared with two currently used products; a low-concentration (TAP-L1) and a high-concentration paste (TAP-H1) mixed in macrogol/propylene glycol vehicle (VEH-1). Sixty-two root canals were infected with E. faecalis biofilm, medicated with (a) TAP-L1, (b) TAP-L2, (c) TAP-H1, (d) VEH-1 or (e) VEH-2 (n = 10) and incubated for 21 days. Live/dead assays were performed using confocal laser scanning microscopy (CLSM), and dead bacteria (%) were calculated. The TAP-H1 group demonstrated the highest dead bacteria (67.54 ± 2.38%), which was significantly higher than the TAP-L2 and TAP-L1 groups (56.85 ± 7.11% and 54.23 ± 10.19%) (p < 0.05). The TAP groups demonstrated significantly higher dead bacteria than the VEH-1 and VEH-2 groups (47.51 ± 6.41% and 45.14 ± 8.28%) (p < 0.05). The ready-to-use TAP-L2 had antibacterial activity comparable to TAP-L1, both of which were lower than TAP-H1.