Enhancing Transfection Efficiency of Spermine-Based Cationic Lipids with a Lysine-Based Spacer
5
Issued Date
2025-01-01
Resource Type
ISSN
18614728
eISSN
1861471X
Scopus ID
2-s2.0-105002612358
Journal Title
Chemistry - An Asian Journal
Rights Holder(s)
SCOPUS
Bibliographic Citation
Chemistry - An Asian Journal (2025)
Suggested Citation
Thongbamrer C., Kunkeaw N., Nguitragool W., Roobsoong W., Sattabongkot J., Pengnam S., Opanasopit P., Yingyongnarongkul B.e. Enhancing Transfection Efficiency of Spermine-Based Cationic Lipids with a Lysine-Based Spacer. Chemistry - An Asian Journal (2025). doi:10.1002/asia.202401751 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/109666
Title
Enhancing Transfection Efficiency of Spermine-Based Cationic Lipids with a Lysine-Based Spacer
Author's Affiliation
Corresponding Author(s)
Other Contributor(s)
Abstract
L-shape spermine-based cationic lipids with a lysine spacer and both identical and non-identical hydrophobic tails were successfully synthesized. Liposomes prepared from these lipids, either alone or in combination with DOPE, demonstrated DNA-binding capability, as confirmed by gel electrophoresis assays. The physicochemical properties, such as size, zeta-potential, and stability of the lipoplexes formed from the cationic lipids were investigated. The liposomes efficiently condensed DNA into compact structures at an N/P ratio of approximately 5–10. Interestingly, liposomes without DOPE exhibited higher transfection efficiency than those containing DOPE, with the cationic lipid featuring a spermine polar head bonded to a lysine spacer and C12–C16 hydrocarbon tails (Sper-Lys-C12,16) achieved the greatest transfection efficiency, as revealed by fluorescence microscopy and flow cytometry. Notably, this formulation maintained high transfection efficiencies even in serum concentrations up to 40%, outperforming the commercial standard Lipofectamine 3000. Additionally, these liposomes exhibited low cytotoxicity, highlighting their potential as safe and effective gene delivery agents.