Temporal shifts in the predominant carbapenemase gene types among carbapenemase-producing Klebsiella pneumoniae isolated in Bangkok, Thailand, during 2013-2016
2
Issued Date
2023-06-01
Resource Type
eISSN
14735644
Scopus ID
2-s2.0-85163907235
Pubmed ID
37294294
Journal Title
Journal of medical microbiology
Volume
72
Issue
6
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of medical microbiology Vol.72 No.6 (2023)
Suggested Citation
Sakamoto N., Laolerd W., Akeda Y., Sugawara Y., Motooka D., Yamamoto N., Takeuchi D., Shanmugakani R.K., Nishi I., Suzuki M., Shibayama K., Iida T., Santanirand P., Tomono K., Hamada S. Temporal shifts in the predominant carbapenemase gene types among carbapenemase-producing Klebsiella pneumoniae isolated in Bangkok, Thailand, during 2013-2016. Journal of medical microbiology Vol.72 No.6 (2023). doi:10.1099/jmm.0.001711 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/87893
Title
Temporal shifts in the predominant carbapenemase gene types among carbapenemase-producing Klebsiella pneumoniae isolated in Bangkok, Thailand, during 2013-2016
Other Contributor(s)
Abstract
Introduction. Carbapenemase-producing Enterobacteriaceae (CPE) have emerged as a global threat to public health and clinical practice.Hypothesis/Gap Statement. In Thailand, reports describing CPEs carrying blaNDM and blaOXA-48-like genes have been increasing recently; however, data on detailed plasmid analysis and temporal shift of sequence type and carbapenemase type are limited.Aim. In this study, we analysed whole-genome sequencing (WGS) data of clinically isolated carbapenemase-producing Klebsiella pneumoniae (CPKP) to reveal the molecular epidemiology of CPKP in a tertiary-care hospital in Bangkok, Thailand.Methodology. Seventy-seven non-duplicated CPKP isolates collected during 2013-2016 were examined for their drug-resistance genes, sequence types and phylogenetic relationships.Results. All the tested isolates possessed carbapenemase gene(s), and the major type of carbapenemase gene in 2014-2015 was blaNDM-1, whereas isolates in 2016 harboured more blaOXA-232 than blaNDM-1. Other carbapenemase gene variants, such as blaNDM-4, blaNDM-5, blaOXA-48, blaOXA-181 and blaIMP-14 were detected in some CPKP isolates. Furthermore, this study revealed that CPKP co-harbouring two genes, blaNDM-1 and blaOXA-232 or blaOXA-181, emerged during this period. Notably, such isolates co-carrying the two carbapenemase genes emerged in three different sequence types, even in a single hospital, and then spread clonally. The WGS of CPKP revealed a temporal shift of the predominant carbapenemase genes from blaNDM-1 to blaOXA-232 along with a variation in other carbapenemase gene types within a span of 4 years.Conclusion. Our findings suggest that a substantial change in CPE types occurred in Thailand and potentially in Southeast Asian countries.