The expression of circulating hsa-miR-126-3p in dengue-infected Thai pediatric patients
Issued Date
2023-01-01
Resource Type
ISSN
20477724
eISSN
20477732
Scopus ID
2-s2.0-85132166391
Pubmed ID
35708203
Journal Title
Pathogens and Global Health
Volume
117
Issue
1
Start Page
76
End Page
84
Rights Holder(s)
SCOPUS
Bibliographic Citation
Pathogens and Global Health Vol.117 No.1 (2023) , 76-84
Suggested Citation
Sriprapun M., Rattanamahaphoom J., Sriburin P., Chatchen S., Limkittikul K., Sirivichayakul C. The expression of circulating hsa-miR-126-3p in dengue-infected Thai pediatric patients. Pathogens and Global Health Vol.117 No.1 (2023) , 76-84. 84. doi:10.1080/20477724.2022.2088465 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/85148
Title
The expression of circulating hsa-miR-126-3p in dengue-infected Thai pediatric patients
Author's Affiliation
Other Contributor(s)
Abstract
Circulating hsa-miRNA-126 (CmiR-126) has been reported to involve in the pathogenesis of many infectious diseases including dengue virus infection. However, no prior study has been conducted to describe more details in dengue-infected pediatric patients. This study aimed to describe CmiR-126-3p in dengue-infected pediatric patients during the febrile and convalescent phases. Additionally, the correlations between CmiR-126-3p and other relevant clinical laboratory factors were investigated. Sixty paired-serum specimens collected during febrile and convalescent phases were retrieved from patients with dengue fever (DF) (n = 30) and dengue hemorrhagic fever (DHF) (n = 30). Thirty paired-serum specimens collected from non-dengue acute febrile illness patients (AFI) were included as the control group. CmiR-126-3p was determined using reverse transcription quantitative real-time polymerase-chain reaction (RT-qPCR). Relative miRNA expression was calculated as 2−ΔCt using CmiR-16-5p for data normalization. CmiR-126-3p expression during febrile and convalescent phases in dengue-infected patients was significantly lower than AFI (p < 0.05). However, miRNA levels were not different (p > 0.05) compared between DF and DHF and between primary and secondary infection. CmiR-126-3p levels in DF in the convalescent were significantly higher than in the febrile phase (p = 0.025). No association between CmiR-126-3p and hematocrit, WBC level, platelet count, WBC differential count or dengue viral load was observed (p > 0.05). The data suggest that hsa-miR-126-3p involved in pathogenesis of dengue infection and may be a promising early and late biomarker for DENV infection. However, hsa-miR-126-3p alone cannot be used as a predictor for dengue severity.