The Detection of SARS-CoV2 Antigen in Wastewater Using an Automated Chemiluminescence Enzyme Immunoassay
Issued Date
2022-07-01
Resource Type
ISSN
16617827
eISSN
16604601
Scopus ID
2-s2.0-85132743292
Pubmed ID
35805441
Journal Title
International Journal of Environmental Research and Public Health
Volume
19
Issue
13
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Environmental Research and Public Health Vol.19 No.13 (2022)
Suggested Citation
Thongpradit S., Prasongtanakij S., Srisala S., Chanprasertyothin S., Pasomsub E., Ongphiphadhanakul B. The Detection of SARS-CoV2 Antigen in Wastewater Using an Automated Chemiluminescence Enzyme Immunoassay. International Journal of Environmental Research and Public Health Vol.19 No.13 (2022). doi:10.3390/ijerph19137783 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/87285
Title
The Detection of SARS-CoV2 Antigen in Wastewater Using an Automated Chemiluminescence Enzyme Immunoassay
Author's Affiliation
Other Contributor(s)
Abstract
The SARS-CoV-2 virus, which is driving the current COVID-19 epidemic, has been detected in wastewater and is being utilized as a surveillance tool to establish an early warning system to aid in the management and prevention of future pandemics. qPCR is the method usually used to detect SARS-CoV-2 in wastewater. There has been no study using an immunoassay that is less laboratory-intensive than qPCR with a shorter turnaround time. Therefore, we aimed to evaluate the performance of an automated chemiluminescence enzyme immunoassay (CLEIA) for SARS-CoV-2 antigen in wastewater. The CLEIA assay achieved 100% sensitivity and 66.7% specificity in a field-captured wastewater sample compared to the gold standard RT-qPCR. Our early findings suggest that the SARS-CoV-2 antigen can be identified in wastewater samples using an automated CLEIA, reducing the turnaround time and improving the performance of SARS-CoV-2 wastewater monitoring during the pandemic.