Abundance of ACVR1B transcript is elevated during septic conditions: Perspectives obtained from a hands-on reductionist investigation
Issued Date
2023-01-01
Resource Type
eISSN
16643224
Scopus ID
2-s2.0-85151792203
Pubmed ID
37020544
Journal Title
Frontiers in Immunology
Volume
14
Rights Holder(s)
SCOPUS
Bibliographic Citation
Frontiers in Immunology Vol.14 (2023)
Suggested Citation
Preechanukul A., Yimthin T., Tandhavanant S., Brummaier T., Chomkatekaew C., Das S., Syed Ahamed Kabeer B., Toufiq M., Rinchai D., West T.E., Chaussabel D., Chantratita N., Garand M. Abundance of ACVR1B transcript is elevated during septic conditions: Perspectives obtained from a hands-on reductionist investigation. Frontiers in Immunology Vol.14 (2023). doi:10.3389/fimmu.2023.1072732 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/81956
Title
Abundance of ACVR1B transcript is elevated during septic conditions: Perspectives obtained from a hands-on reductionist investigation
Author's Affiliation
Faculty of Tropical Medicine, Mahidol University
Mahidol Oxford Tropical Medicine Research Unit
Sidra Medicine
University of Washington School of Medicine
Universitat Basel
Swiss Tropical and Public Health Institute (Swiss TPH)
Washington University School of Medicine in St. Louis
University of Washington
Mahidol Oxford Tropical Medicine Research Unit
Sidra Medicine
University of Washington School of Medicine
Universitat Basel
Swiss Tropical and Public Health Institute (Swiss TPH)
Washington University School of Medicine in St. Louis
University of Washington
Other Contributor(s)
Abstract
Sepsis is a complex heterogeneous condition, and the current lack of effective risk and outcome predictors hinders the improvement of its management. Using a reductionist approach leveraging publicly available transcriptomic data, we describe a knowledge gap for the role of ACVR1B (activin A receptor type 1B) in sepsis. ACVR1B, a member of the transforming growth factor-beta (TGF-beta) superfamily, was selected based on the following: 1) induction upon in vitro exposure of neutrophils from healthy subjects with the serum of septic patients (GSE49755), and 2) absence or minimal overlap between ACVR1B, sepsis, inflammation, or neutrophil in published literature. Moreover, ACVR1B expression is upregulated in septic melioidosis, a widespread cause of fatal sepsis in the tropics. Key biological concepts extracted from a series of PubMed queries established indirect links between ACVR1B and “cancer”, “TGF-beta superfamily”, “cell proliferation”, “inhibitors of activin”, and “apoptosis”. We confirmed our observations by measuring ACVR1B transcript abundance in buffy coat samples obtained from healthy individuals (n=3) exposed to septic plasma (n = 26 melioidosis sepsis cases)ex vivo. Based on our re-investigation of publicly available transcriptomic data and newly generated ex vivo data, we provide perspective on the role of ACVR1B during sepsis. Additional experiments for addressing this knowledge gap are discussed.