Luciferin Synthesis and Pesticide Detection by Luminescence Enzymatic Cascades
Issued Date
2022-04-11
Resource Type
ISSN
14337851
eISSN
15213773
Scopus ID
2-s2.0-85124903095
Pubmed ID
35138676
Journal Title
Angewandte Chemie - International Edition
Volume
61
Issue
16
Rights Holder(s)
SCOPUS
Bibliographic Citation
Angewandte Chemie - International Edition Vol.61 No.16 (2022)
Suggested Citation
Watthaisong P., Kamutira P., Kesornpun C., Pongsupasa V., Phonbuppha J., Tinikul R., Maenpuen S., Wongnate T., Nishihara R., Ohmiya Y., Chaiyen P. Luciferin Synthesis and Pesticide Detection by Luminescence Enzymatic Cascades. Angewandte Chemie - International Edition Vol.61 No.16 (2022). doi:10.1002/anie.202116908 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84095
Title
Luciferin Synthesis and Pesticide Detection by Luminescence Enzymatic Cascades
Other Contributor(s)
Abstract
D-Luciferin (D-LH2), a substrate of firefly luciferase (Fluc), is important for a wide range of bioluminescence applications. This work reports a new and green method using enzymatic reactions (HELP, HadA Enzyme for Luciferin Preparation) to convert 19 phenolic derivatives to 8 D-LH2 analogues with ≈51 % yield. The method can synthesize the novel 5′-methyl-D-LH2 and 4′,5′-dimethyl-D-LH2, which have never been synthesized or found in nature. 5′-Methyl-D-LH2 emits brighter and longer wavelength light than the D-LH2. Using HELP, we further developed LUMOS (Luminescence Measurement of Organophosphate and Derivatives) technology for in situ detection of organophosphate pesticides (OPs) including parathion, methyl parathion, EPN, profenofos, and fenitrothion by coupling the reactions of OPs hydrolase and Fluc. The LUMOS technology can detect these OPs at parts per trillion (ppt) levels. The method can directly detect OPs in food and biological samples without requiring sample pretreatment.