Combined effect of brazilin-rich extract and lawsone methyl ether against infection-causing bacteria
Issued Date
2024-06-01
Resource Type
ISSN
1319562X
Scopus ID
2-s2.0-85190462834
Journal Title
Saudi Journal of Biological Sciences
Volume
31
Issue
6
Rights Holder(s)
SCOPUS
Bibliographic Citation
Saudi Journal of Biological Sciences Vol.31 No.6 (2024)
Suggested Citation
Nirmal N., Koirala P., Khanashyam A.C., Panichayupakaranant P., Septama A.W. Combined effect of brazilin-rich extract and lawsone methyl ether against infection-causing bacteria. Saudi Journal of Biological Sciences Vol.31 No.6 (2024). doi:10.1016/j.sjbs.2024.103999 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98074
Title
Combined effect of brazilin-rich extract and lawsone methyl ether against infection-causing bacteria
Corresponding Author(s)
Other Contributor(s)
Abstract
Bacterial contamination and infection widely affect the food, pharmaceutical and biomedical industries. Additionally, these bacteria developed resistance to synthetic antibiotics causing public health danger, globally. Natural plant extracts (NPE) are suitable alternatives to synthetic antibiotics to tackle antimicrobial resistance problems. Furthermore, a blend or combination of different NPEs exerts a wide spectrum of antimicrobial activity. Therefore, the combined effect of brazilin-rich extract (BRE) and lawsome methyl ether (LME) against infection-causing common bacteria were evaluated. BRE had a lower minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against most of the Gram-negative bacteria (Salmonella typhi, Salmonella typhimurium and Pseudomonas aeruginosa) while LME was active against most of the Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus, and Staphylococcus epidermidis). The combination of BRE and LME at 2:1 and 1:1 concentration significantly reduced the MIC value of each compound as compared to either BRE or LME concentration alone (P < 0.05). Further time-kill kinetics revealed a 3.0–3.5 log reduction in Gram-positive bacteria and a 2.5–3.0 log reduction in Gram-negative bacteria during 120 min of incubation, respectively. Therefore, a combination of BRE and LME was recommended as natural antibacterial to synthetic antibiotics for food and pharmaceutical applications.