Anti-CAMP1 IgG promotes macrophage phagocytosis of Cutibacterium acnes type II
Issued Date
2024-08-01
Resource Type
ISSN
09445013
Scopus ID
2-s2.0-85193296570
Journal Title
Microbiological Research
Volume
285
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microbiological Research Vol.285 (2024)
Suggested Citation
Min T.T., Choowongkomon K., Htoo H.H., Nonejuie P., Haltrich D., Yamabhai M. Anti-CAMP1 IgG promotes macrophage phagocytosis of Cutibacterium acnes type II. Microbiological Research Vol.285 (2024). doi:10.1016/j.micres.2024.127749 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/98440
Title
Anti-CAMP1 IgG promotes macrophage phagocytosis of Cutibacterium acnes type II
Corresponding Author(s)
Other Contributor(s)
Abstract
Among 5 types of the Christie-Atkins-Munch-Petersen factor (CAMP) of Cutibacterium acnes, CAMP1 is highly expressed in phylotype II as well as IB, and thought to be a virulence factor of opportunistic but fatal blood, soft tissue, and implant-related infections. The target of a human single-chain variable antibody fragment (scFv), recently isolated from a phage display library, has been identified as CAMP1 of phylotype II, using immunoprecipitation followed by mass spectrometry, phage display peptide biopanning, 3D-modelling, and ELISA. The IgG1 format of the antibody could enhance phagocytosis of C. acnes DMST 14916 by THP-1 human monocytes. Our results suggest that the antibody-dependent phagocytosis process is mediated by the caveolae membrane system and involves the induction of IL-1β. This is the first report on the study of a human antibody against CAMP1 of C. acnes phylotype II, of which a potential use as therapeutic antibody against virulence C. acnes infection is postulated.