Stability evaluations on Thunbergia laurifolia Lindl. leaf extract through HPLC, TLC and free radical scavenging activity analysis
Issued Date
2019
Resource Type
Language
eng
Rights
Mahidol University
Rights Holder(s)
Faculty of Pharmacy Mahidol University
Suggested Citation
Chaweewan Suwanvecho, Pongtip Sithisarn, Supavadee Timum, Nidthida Archeepsudjarit, Piyanuch Rojsanga, ปิยนุช โรจน์สง่า (2019). Stability evaluations on Thunbergia laurifolia Lindl. leaf extract through HPLC, TLC and free radical scavenging activity analysis. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/52389
Title
Stability evaluations on Thunbergia laurifolia Lindl. leaf extract through HPLC, TLC and free radical scavenging activity analysis
Abstract
Regulatory guidelines recommend efficient stability studies on herbal products to
establish their shelf lifes. In this study, dried extracts of Thunbergia laurifolia Lindl. leaves
were subjected to three different storage conditions for six months: 1) 30 ± 2 ºC, 75 ± 5 %
relative humidity (RH), 2) 40 ± 2 ºC, 75 ± 5 %RH, and 3) 2-8 ºC. Control and stability tested
samples were evaluated for physical appearance, moisture content, caffeic acid and rosmarinic
acid contents as chemical markers, and thin layer chromatography fingerprints, and
determination for DPPH scavenging activity. Chemical marker contents and DPPH scavenging
activities of the stability tested samples comparably retained with respect to the control samples
after the storage for 3 months in all storage conditions. Pearson correlation analysis revealed
the strong positive correlation between antioxidant activity and rosmarinic acid content of
stability samples stored after 6 months with correlation coefficient (r) values more than 0.901
However, antioxidant activity was correlated positively with the caffeic acid content only
stored after 3 months (r values less than 0.742). The results suggested that rosmarinic acid is
more suitable to use as a chemical marker for evaluation of chemical and biological shelf lives
of T. laurifolia leaf products.
Description
The 1st Pharmaceutical Sciences Asia Conference 2019 Theme : Pharmaceutical Sciences toward Health Innovation in the Disruptive Era. Bangkok Midtown Hotel, Thailand. August 22, 2019, page 40-44