Stimulation of adenosine A<inf>1</inf> receptor prevents oxidative injury in H9c2 cardiomyoblasts: Role of Gβγ-mediated Akt and ERK1/2 signaling
Issued Date
2022-09-15
Resource Type
ISSN
0041008X
eISSN
10960333
Scopus ID
2-s2.0-85137008974
Pubmed ID
35901927
Journal Title
Toxicology and Applied Pharmacology
Volume
451
Rights Holder(s)
SCOPUS
Bibliographic Citation
Toxicology and Applied Pharmacology Vol.451 (2022)
Suggested Citation
Mangmool S., Kyaw E.T.H., Nuamnaichati N., Pandey S., Parichatikanond W. Stimulation of adenosine A<inf>1</inf> receptor prevents oxidative injury in H9c2 cardiomyoblasts: Role of Gβγ-mediated Akt and ERK1/2 signaling. Toxicology and Applied Pharmacology Vol.451 (2022). doi:10.1016/j.taap.2022.116175 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86837
Title
Stimulation of adenosine A<inf>1</inf> receptor prevents oxidative injury in H9c2 cardiomyoblasts: Role of Gβγ-mediated Akt and ERK1/2 signaling
Author's Affiliation
Other Contributor(s)
Abstract
Oxidative stress causes cellular injury and damage in the heart primarily through apoptosis resulting in cardiac abnormalities such as heart failure and cardiomyopathy. During oxidative stress, stimulation of adenosine receptor (AR) has been shown to protect against oxidative damage due to their cytoprotective properties. However, the subtype specificity and signal transductions of adenosine A1 receptor (A1R) on cardiac protection during oxidative stress have remained elusive. In this study, we found that stimulation of A1Rs with N6-cyclopentyladenosine (CPA), a specific A1R agonist, attenuated the H2O2-induced intracellular and mitochondrial reactive oxygen species (ROS) production and apoptosis. In addition, A1R stimulation upregulated the synthesis of antioxidant enzymes (catalase and GPx-1), antiapoptotic proteins (Bcl-2 and Bcl-xL), and mitochondria-related markers (UCP2 and UCP3). Blockades of Gβγ subunit of heterotrimeric Gαi protein antagonized A1R-mediated antioxidant and antiapoptotic effects, confirming the potential role of Gβγ subunit-mediated A1R signaling. Additionally, cardioprotective effects of CPA mediated through PI3K/Akt- and ERK1/2-dependent signaling pathways. Thus, we propose that A1R represents a promising therapeutic target for prevention of oxidative injury in the heart.