Effect of platelet-rich plasma and other key factors on hair follicle preservation
Issued Date
2023-03-01
Resource Type
ISSN
09747753
eISSN
09749241
Scopus ID
2-s2.0-85168369567
Journal Title
International Journal of Trichology
Volume
15
Issue
2
Start Page
50
End Page
55
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Trichology Vol.15 No.2 (2023) , 50-55
Suggested Citation
Thuangtong R., Chaweekulrat P., Thanomkitti K., Triwongwaranat D., Rujitharanawong C. Effect of platelet-rich plasma and other key factors on hair follicle preservation. International Journal of Trichology Vol.15 No.2 (2023) , 50-55. 55. doi:10.4103/ijt.ijt_65_22 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/88866
Title
Effect of platelet-rich plasma and other key factors on hair follicle preservation
Author's Affiliation
Other Contributor(s)
Abstract
Background: Hair graft preservation is an important factor that influences graft survival in hair transplantation. Objective: To investigate the benefits of adding platelet-rich plasma (PRP), and the effect of different storage solutions and temperatures on hair follicle preservation. Materials and Methods: This randomized-controlled study included 10 androgenetic alopecia patients who underwent hair transplantation. Forty-five hair grafts were collected from each patient and then randomized to 8 different culture conditions for 7 days. Hair grafts were cultured in Williams' Medium E or Ringer's lactate solution (RLS) at either 4°C or 37°C, and with or without 10% PRP supplementation. Results: In vitro hair growth in Williams' Medium E was significantly greater than in RLS. The 37°C temperature condition was found to be significantly better than the 4°C condition. The growth of hair grafts cultured with PRP was not significance difference from those without PRP. However, immunofluorescence staining for cytokeratin 15 showed greater expression in hair graft cultured with PRP. Conclusion: PRP may have a beneficial effect for preserving the viability of hair grafts. Williams' Medium E and 37°C temperature were found to be superior to RLS and 4°C relative to hair follicle growth in organ culture.