Burkholderia pseudomallei pathogenesis in human skin fibroblasts: A Bsa type III secretion system is involved in the invasion, multinucleated giant cell formation, and cellular damage
1
Issued Date
2022-02-01
Resource Type
eISSN
19326203
Scopus ID
2-s2.0-85123973080
Pubmed ID
35113856
Journal Title
PLoS ONE
Volume
17
Issue
2 February
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE Vol.17 No.2 February (2022)
Suggested Citation
Kaewpan A., Duangurai T., Rungruengkitkun A., Muangkaew W., Kanjanapruthipong T., Jitprasutwit N., Ampawong S., Sukphopetch P., Chantratita N., Pumirat P. Burkholderia pseudomallei pathogenesis in human skin fibroblasts: A Bsa type III secretion system is involved in the invasion, multinucleated giant cell formation, and cellular damage. PLoS ONE Vol.17 No.2 February (2022). doi:10.1371/journal.pone.0261961 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/86623
Title
Burkholderia pseudomallei pathogenesis in human skin fibroblasts: A Bsa type III secretion system is involved in the invasion, multinucleated giant cell formation, and cellular damage
Author's Affiliation
Other Contributor(s)
Abstract
Burkholderia pseudomallei-a causative agent of melioidosis that is endemic in Southeast Asia and Northern Australia-is a Gram-negative bacterium transmitted to humans via inhalation, inoculation through skin abrasions, and ingestion. Melioidosis causes a range of clinical presentations including skin infection, pneumonia, and septicemia. Despite skin infection being one of the clinical symptoms of melioidosis, the pathogenesis of B. pseudomallei in skin fibroblasts has not yet been elucidated. In this study, we investigated B. pseudomallei pathogenesis in the HFF-1 human skin fibroblasts. On the basis of co-culture assays between different B. pseudomallei clinical strains and the HFF-1 human skin fibroblasts, we found that all B. pseudomallei strains have the ability to mediate invasion, intracellular replication, and multinucleated giant cell (MNGC) formation. Furthermore, all strains showed a significant increase in cytotoxicity in human fibroblasts, which coincides with the augmented expression of matrix metalloproteinase-2. Using B. pseudomallei mutants, we showed that the B. pseudomallei Bsa type III secretion system (T3SS) contributes to skin fibroblast pathogenesis, but O-polysaccharide, capsular polysaccharide, and short-chain dehydrogenase metabolism do not play a role in this process. Taken together, our findings reveal a probable connection for the Bsa T3SS in B. pseudomallei infection of skin fibroblasts, and this may be linked to the pathogenesis of cutaneous melioidosis.