Preparation and characterization of antibodies against reassortant rotavirus strain RV441
1
Issued Date
2024
Copyright Date
1989
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
viii, 186 leaves. : ill.
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Microbiology))--Mahidol University, 1989
Suggested Citation
Tasanee Tengchaisri Preparation and characterization of antibodies against reassortant rotavirus strain RV441. Thesis (M.Sc. (Microbiology))--Mahidol University, 1989. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/100108
Title
Preparation and characterization of antibodies against reassortant rotavirus strain RV441
Alternative Title(s)
การเตรียมและศึกษาคุณสมบัติของแอนติบอดีย์ต่อโรต้าไวรัส สายพันธุ์ผสม
Author(s)
Abstract
Rotaviruses have been shown to be the major cause of infantile gastroenteritis in developed and developing countries. In developing countries, the serological assays are the main use of rotavirus detection but the commercially available reagents used for the assays are too expensive. The reference rotavirus antigens and antibodies representative of subgroups and serotypes are not yet available. A reassorthant rotavirus strain RV441 with subgroup I serotype 2 antigenic determinants was propagated in MA104 cells, precipitated with polyethyleneglycol, extracted with fluorocarbon and then used to immunize rabbit. Hyperimmune reaponses of rabbit was detected by ELISA. Immunoblotting analysis of rabbit antiserum showed that immune response directed strongly to VP2 and VP5 and weakly to VP6 and Vp7 structural proteins of rotavirus. Purified immunoglobulin was prepared form rabbit antiserum and used at the optimal dilutions to develop a double-sandwich ELISA to monitor the persence of rotavirus in clinical spectimens. A total of 260 fecal specimens from diarrheic patients were tested. Only 88 specimens showed high OD(,490) reading value (1.12 - 2.5), and all of them were short RNA electropherotypes. Other 12 specimens reacted weakly in the ELISA test (OD (,490) = 0.23 - 1.00), and had been confirmed possitive by blobking ELISA test. The remaining 160 specimens, composed of 100 specimens with long electropherotype and 60 specimens negative for rotavirus, all showed negative results in this ELISA. We proposed that this ELISA system may be of value in identifying subgroup I human rotavirus but further test of additional clinical specimens and other reference rotavirus strains are required to confirm the sensitivity and specificity of the method.
Description
Microbiology (Mahidol University 1989)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Microbiology
Degree Grantor(s)
Mahidol University