Intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein
Issued Date
2022-10-01
Resource Type
ISSN
24058440
Scopus ID
2-s2.0-85139351559
Journal Title
Heliyon
Volume
8
Issue
10
Rights Holder(s)
SCOPUS
Bibliographic Citation
Heliyon Vol.8 No.10 (2022)
Suggested Citation
Klincumhom N., Lorthongpanich C., Thumanu K., Septham P., Phomyu W., Issaragrisil S., Pavasant P. Intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein. Heliyon Vol.8 No.10 (2022). doi:10.1016/j.heliyon.2022.e10845 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86468
Title
Intermittent compressive force regulates human periodontal ligament cell behavior via yes-associated protein
Other Contributor(s)
Abstract
Intermittent compressive force influences human periodontal ligament (PDL) cell behavior that facilitates periodontal tissue regeneration. In response to mechanical stimuli, Yes-associated protein (YAP) has been recognized as a mechanosensitive transcriptional activator that regulates cell proliferation and cell fate decisions. This study aimed to investigate whether compressive forces influence cell proliferation and cell fate decisions of human PDL cells via YAP signaling. YAP expression was silenced by shRNA. The effect of YAP on cell proliferation, adipogenesis and osteogenesis of PDL cells under ICF loading were determined. Adipogenic differentiation bias upon ICF loading was confirmed by fourier-transform infrared spectroscopy (FTIR). The results revealed that ICF-induced YAP promotes osteogenesis, but it inhibits adipogenesis in PDL cells. Depletion of YAP results in PDL cells that are irresponsive to ICF and, therefore, the failure of the PDL cells to undergo osteogenic differentiation. This was shown by a significant reduction in calcium deposited in the CF-derived osteoblasts of the YAP-knockdown (YAP-KD) PDL cells. As to control treatment, reduction of YAP promoted adipogenesis, whereas ICF-induced YAP inhibited this mechanism. However, the adipocyte differentiation in YAP-KD cells was not affected upon ICF treatment as the YAP-KD cells still exhibited a better adipogenic differentiation that was unrelated to the ICF. This study demonstrated that, in response to ICF treatment, YAP could be a crucial mechanosensitive transcriptional activator for the regulation of PDL cell behavior through a mechanobiological process. Our results may provide the possibility of facilitating PDL tissue regeneration by manipulation of the Hippo-YAP signaling pathway.