Performance Evaluation of Focal Plane Array (FPA)-FTIR and Synchrotron Radiation (SR)-FTIR Microspectroscopy to Classify Rice Components
Issued Date
2022-12-06
Resource Type
ISSN
14319276
eISSN
14358115
Scopus ID
2-s2.0-85142212385
Journal Title
Microscopy and Microanalysis
Volume
28
Issue
6
Start Page
2059
End Page
2068
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microscopy and Microanalysis Vol.28 No.6 (2022) , 2059-2068
Suggested Citation
Siriwong S., Tanthanuch W., Srisamut D., Chantarakhon C., Kamkajon K., Thumanu K. Performance Evaluation of Focal Plane Array (FPA)-FTIR and Synchrotron Radiation (SR)-FTIR Microspectroscopy to Classify Rice Components. Microscopy and Microanalysis Vol.28 No.6 (2022) , 2059-2068. 2068. doi:10.1017/S1431927622012454 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/86904
Title
Performance Evaluation of Focal Plane Array (FPA)-FTIR and Synchrotron Radiation (SR)-FTIR Microspectroscopy to Classify Rice Components
Author's Affiliation
Other Contributor(s)
Abstract
The development of biochemical analysis techniques to study heterogeneous biological samples is increasing. These techniques include synchrotron radiation Fourier transform infrared (SR-FTIR) microspectroscopy. This method has been applied to analyze biological tissue with multivariate statistical analysis to classify the components revealed by the spectral data. This study aims to compare the efficiencies of SR-FTIR microspectroscopy and focal plane array (FPA)-FTIR microspectroscopy when classifying rice tissue components. Spectral data were acquired for mapping the same sample areas from both techniques. Principal component analysis and cluster imaging were used to investigate the biochemical variations of the tissue types. The classification was based on the functional groups of pectin, protein, and polysaccharide. Four layers from SR-FTIR microspectroscopy including pericarp, aleurone layer, sub-aleurone layer, and endosperm were classified using cluster imaging, while FPA-FTIR microspectroscopy could classify only three layers of pericarp, aleurone layer, and endosperm. Moreover, SR-FTIR microspectroscopy increased the image contrast of the biochemical distribution in rice tissue more efficiently than FPA-FTIR microspectroscopy. We have demonstrated the capability of the high-resolution synchrotron technique and its ability to clarify small structures in rice tissue. The use of this technique might increase in future studies of tissue characterization.
