Shrimp Vago5 activates an innate immune defense upon bacterial infection
Issued Date
2022-01-01
Resource Type
ISSN
10504648
eISSN
10959947
Scopus ID
2-s2.0-85119274500
Pubmed ID
34742902
Journal Title
Fish and Shellfish Immunology
Volume
120
Start Page
122
End Page
132
Rights Holder(s)
SCOPUS
Bibliographic Citation
Fish and Shellfish Immunology Vol.120 (2022) , 122-132
Suggested Citation
Boonchuen P., Sakhor H., Jaree P., Somboonwiwat K. Shrimp Vago5 activates an innate immune defense upon bacterial infection. Fish and Shellfish Immunology Vol.120 (2022) , 122-132. 132. doi:10.1016/j.fsi.2021.10.044 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/83399
Title
Shrimp Vago5 activates an innate immune defense upon bacterial infection
Author(s)
Author's Affiliation
Other Contributor(s)
Abstract
Acute hepatopancreatic necrosis disease, AHPND, caused by a specific Vibrio parahaemolyticus (VPAHPND) strain, results in a great loss of global shrimp production. This study performed suppression subtractive hybridization (SSH) to identify differentially expressed genes from white shrimp Penaeus vannamei hemocyte upon VPAHPND infection. Among the immune-related genes identified, Vago5, kunitz, secretory leukocyte proteinase inhibitor, and profilin are the most abundant genes classified as the up-regulated genes in the SSH library. The qRT-PCR results show that only Vago5 was highly up-regulated at 3 and 6 h post-VPAHPND challenge, whereas kunitz, secretory leukocyte proteinase inhibitor, and profilin were highly up-regulated at 48 h post-VPAHPND challenge. As an early VPAHPND infection-responsive gene, Vago5 was further functional characterized by RNA interference. Knockdown of Vago5 gene resulted in the significantly rapid increase of shrimp mortality and the number of bacteria in the stomach and hepatopancreas upon VPAHPND infection. Moreover, downstream genes of Toll, IMD, and JAK/STAT pathways and phenoloxidase system were analyzed for the expression in the VPAHPND-infected shrimp hemocyte after dsVago5 treatment. Vago5 gene knockdown resulted in a significant decrease in transcript levels of PEN4, TNF, and PO2 genes as well as PO activity in the hemolymph, suggesting that Vago5 might modulate antibacterial infection through activation of the genes in the NF-κB mediated pathways, JAK/STAT pathway, and phenoloxidase system.