Aptamer-Based Label-Free Colorimetric Assay Using Gold Nanoparticles for Specific Detection of Streptococcus suis
Issued Date
2026-04-01
Resource Type
eISSN
20796374
Scopus ID
2-s2.0-105036730073
Journal Title
Biosensors
Volume
16
Issue
4
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biosensors Vol.16 No.4 (2026)
Suggested Citation
Sangboonruang S., Semakul N., Matchawong A., Sattaphan A., Saengsawang K., Srisawat C., Tragoolpua K., Tharinjaroen C.S. Aptamer-Based Label-Free Colorimetric Assay Using Gold Nanoparticles for Specific Detection of Streptococcus suis. Biosensors Vol.16 No.4 (2026). doi:10.3390/bios16040215 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/116470
Title
Aptamer-Based Label-Free Colorimetric Assay Using Gold Nanoparticles for Specific Detection of Streptococcus suis
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Abstract
Streptococcus suis is a serious zoonotic pathogen responsible for rapid progression and deadly infections in both humans and pigs. With an increasing number of reported cases and considering the limitations of standard routine identification, a simple, rapid, and cost-effective approach is needed. In this study, a label-free colorimetric assay based on gold nanoparticles (AuNPs) was applied with a specific aptamer, R8-su12. This assay offered simplified detection through observable color change, enabling visual analysis by the naked eye or assessment via UV–Vis spectrophotometry. Under the optimal assay conditions, the detection procedure was carried out within 45 min. The reaction of the aptasensor and other bacterial species, including Staphylococcus aureus, S. pneumoniae, S. pyogenes, Pseudomonas aeruginosa, Escherichia coli, Enterococcus faecium, and E. faecalis, was not present, indicating the specificity of this assay. Moreover, the aptasensor exhibited high sensitivity with a limit of detection (LOD) at 1 CFU of S. suis and had broad reactivity with S. suis serotypes 1, 1/2, 9, and 14, as well as with S. suis isolated from clinical specimens. Thus, this aptasensor demonstrates proof-of-concept feasibility including clinical sample testing before practical implementation. It holds promise as a practical tool for the early screening and outbreak management of S. suis in a variety of settings, such as clinical laboratories, food safety, and the environment.