Up-regulation of microRNA 101-3p during erythropoiesis in β-thalassemia/HbE
6
Issued Date
2023-11-01
Resource Type
ISSN
10799796
eISSN
10960961
Scopus ID
2-s2.0-85165256583
Journal Title
Blood Cells, Molecules, and Diseases
Volume
103
Rights Holder(s)
SCOPUS
Bibliographic Citation
Blood Cells, Molecules, and Diseases Vol.103 (2023)
Suggested Citation
Phannasil P., Sukhuma C., Nauphar D., Nuamsee K., Svasti S. Up-regulation of microRNA 101-3p during erythropoiesis in β-thalassemia/HbE. Blood Cells, Molecules, and Diseases Vol.103 (2023). doi:10.1016/j.bcmd.2023.102781 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/88134
Title
Up-regulation of microRNA 101-3p during erythropoiesis in β-thalassemia/HbE
Author(s)
Other Contributor(s)
Abstract
Ineffective erythropoiesis is the main cause of anemia in β-thalassemia. The crucial hallmark of ineffective erythropoiesis is the high proliferation of erythroblast. microRNA (miR/miRNA) involves several biological processes, including cell proliferation and erythropoiesis. miR-101 was widely studied and associated with proliferation in several types of cancer. However, the miR-101-3p has not been studied in β-thalassemia/HbE. Therefore, this study aims to investigate the expression of miR-101-3p during erythropoiesis in β-thalassemia/HbE. The results showed that miR-101-3p was upregulated in the erythroblast of β-thalassemia/HbE patients on day 7, indicating that miR-101-3p may be involved with high proliferation in β-thalassemia/HbE. Therefore, the mRNA targets of miR-101-3p including Rac1, SUB1, TET2, and TRIM44 were investigated to determine the mechanisms involved with high proliferation of β-thalassemia/HbE erythroblasts. Rac1 expression was significantly reduced at day 11 in severe β-thalassemia/HbE compared to normal controls and mild β-thalassemia/HbE. SUB1 gene expression was significantly lower in severe β-thalassemia/HbE compared to normal controls at day 9 of culture. For TET2 and TRIM44 expression, a significant difference was not observed among normal and β-thalassemia/HbE. However, the high expression of miR-101-3p at day 7 and these target genes was not correlated, suggesting that this miRNA may regulate ineffective erythropoiesis in β-thalassemia/HbE via other target genes.