GC–MS analysis of an essential oil blend and its prevention of human fibroblast cells undergoing cell aging after exposure to UVB irradiation
Issued Date
2026-08-01
Resource Type
eISSN
26670313
Scopus ID
2-s2.0-105040605917
Journal Title
Phytomedicine Plus
Volume
6
Issue
3
Rights Holder(s)
SCOPUS
Bibliographic Citation
Phytomedicine Plus Vol.6 No.3 (2026)
Suggested Citation
Saiyudthong S., Rayasilp K., Ratanachamnong P., Rungsiwiwut R., Samatiwat P., Jaisin Y. GC–MS analysis of an essential oil blend and its prevention of human fibroblast cells undergoing cell aging after exposure to UVB irradiation. Phytomedicine Plus Vol.6 No.3 (2026). doi:10.1016/j.phyplu.2026.100999 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/117184
Title
GC–MS analysis of an essential oil blend and its prevention of human fibroblast cells undergoing cell aging after exposure to UVB irradiation
Corresponding Author(s)
Other Contributor(s)
Abstract
Skin aging is influenced by environmental factors, particularly exposure to ultraviolet (UV) radiation from sunlight, which contributes to the development of wrinkles and fine lines. Essential oils, which are rich in bioactive compounds with antioxidant properties, have attracted interest for their potential role in skin protection and the attenuation of aging-related changes. This study examined the protective effects of Ylang-Ylang, Damask rose, and Chamomile essential oils, individually and in combination, against UVB-induced cytotoxicity and aging in human fibroblast cells. Pretreatment with each essential oil attenuated UVB-induced cytotoxicity and reduced intracellular oxidative stress in fibroblast cells, as demonstrated by cell viability and ROS assays. The essential oil blend showed a similar trend, with slightly higher cell survival and lower ROS levels observed under the same conditions. In addition, MMP-1 levels were decreased, while collagen type I expression showed partial recovery compared with UVB exposure alone. The increased effect observed with the blend may reflect a combined action of its constituents, although this requires further investigation. GC–MS analysis identified a relative range of compounds that may contribute to these effects. Altogether, these findings suggest that the essential oil blend may have potential for skin cell protection and anti-aging effects in UVB-irradiated fibroblast cells, possibly through antioxidant-related mechanisms. This study provides a preliminary understanding of the biological effects of the essential oil blend in an in vitro model. However, further research is needed to clarify the underlying mechanisms and to confirm its efficacy in an in vivo model.