Practical Methods to Permit the Analysis of Host Biomarkers in Resource-Limited Settings
Issued Date
2022-06-01
Resource Type
ISSN
00029637
eISSN
14761645
Scopus ID
2-s2.0-85132765425
Pubmed ID
35436761
Journal Title
American Journal of Tropical Medicine and Hygiene
Volume
106
Issue
6
Start Page
1765
End Page
1769
Rights Holder(s)
SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene Vol.106 No.6 (2022) , 1765-1769
Suggested Citation
Chandna A., Richard-Greenblatt M., Tustin R., Lee S.J., Kain K.C., Burza S., Lubell Y., Turner P. Practical Methods to Permit the Analysis of Host Biomarkers in Resource-Limited Settings. American Journal of Tropical Medicine and Hygiene Vol.106 No.6 (2022) , 1765-1769. 1769. doi:10.4269/ajtmh.21-1045 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/84975
Title
Practical Methods to Permit the Analysis of Host Biomarkers in Resource-Limited Settings
Other Contributor(s)
Abstract
Host biomarker testing can be used as an adjunct to the clinical assessment of patients with infections and might be particularly impactful in resource-constrained settings. Research on the merits of this approach at peripheral levels of low- and middle-income country health systems is limited. In part, this is due to resource-intense requirements for sample collection, processing, and storage. We evaluated the stability of 16 endothelial and immune activation biomarkers implicated in the host response to infection stored in venous plasma and dried blood spot specimens at different temperatures for 6 months. We found that -80̊C storage offered no clear advantage over -20̊C for plasma aliquots, and most biomarkers studied could safely be stored as dried blood spots at refrigeration temperatures (4̊C) for up to 3 months. These results identify more practical methods for host biomarker testing in resource-limited environments, which could help facilitate research in rural and remote environments.