Development of an immunochromatographic strip test for rapid detection of a potent neutralizing anti-interferon gamma antibody in adult-onset immunodeficiency
Issued Date
2026-03-01
Resource Type
ISSN
0125877X
Scopus ID
2-s2.0-105036044712
Pubmed ID
37578481
Journal Title
Asian Pacific Journal of Allergy and Immunology
Volume
44
Issue
1
Start Page
211
End Page
216
Rights Holder(s)
SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology Vol.44 No.1 (2026) , 211-216
Suggested Citation
Yasamut U., Thongkum W., Wongsawat E., Tayapiwatana C. Development of an immunochromatographic strip test for rapid detection of a potent neutralizing anti-interferon gamma antibody in adult-onset immunodeficiency. Asian Pacific Journal of Allergy and Immunology Vol.44 No.1 (2026) , 211-216. 216. doi:10.12932/AP-010523-1597 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/116376
Title
Development of an immunochromatographic strip test for rapid detection of a potent neutralizing anti-interferon gamma antibody in adult-onset immunodeficiency
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Author's Affiliation
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Abstract
BACKGROUND: Adult-onset immunodeficiency (AOID) is associated with the presence of anti-interferon gamma autoantibodies (anti-IFN-γ autoAbs), which neutralizes IFN-γ signaling and leads to susceptibility to intracellular opportunistic infections. However, measuring neutralizing autoAbs is not practical for general clinical laboratories. OBJECTIVE: This study aims to develop a competitive immunochromatographic (IC) strip test for detecting autoAbs that recognize the same epitope as a potent neutralizing antibody. METHODS: The competitive IC strip test detecting autoAbs that recognize the same epitope as mouse anti-IFN-γ monoclonal antibody, clone B27 (B27 mAb) was fabricated and used to determine B27 epitope recognizing autoAb (B27 AAb) in AOID plasma. The competitive ELISA was used as a comparative method. AR patients. RESULTS: The efficacy of the IC strip test was compared with competitive ELISA and found a percent positive agreement of 91.30%, a percent negative agreement of 79.31%, and a percent overall agreement of 84.62%. CONCLUSIONS: The results from the competitive IC strip test were consistent with those from competitive ELISA, indicating that the generated IC strip could detect B27 AAb in AOID plasma.