Radiosynthesis of Novel<sup>68</sup>Ga-labeled α-Cyclodextrin for PET Imaging
Issued Date
2024-01-01
Resource Type
ISSN
08574421
eISSN
2586940X
Scopus ID
2-s2.0-85189241316
Journal Title
Journal of Health Research
Volume
38
Start Page
S39
End Page
S47
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Health Research Vol.38 (2024) , S39-S47
Suggested Citation
Sriaumpai S., Shiratori S., Denariyakoon S., Chatamra K., Jansook P. Radiosynthesis of Novel<sup>68</sup>Ga-labeled α-Cyclodextrin for PET Imaging. Journal of Health Research Vol.38 (2024) , S39-S47. S47. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/97911
Title
Radiosynthesis of Novel<sup>68</sup>Ga-labeled α-Cyclodextrin for PET Imaging
Author's Affiliation
Corresponding Author(s)
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Abstract
Background: Cyclodextrins (CDs) are cyclic oligosaccharides composed of glucose units linked by α-1, 4 glycosidic bond. Recently, CDs have been promising for the application of medical imaging, especially nuclear medicine imaging. Herein, we aim to simply radiosynthesis68Ga-alpha-cyclodextrin (68Ga-αCD) by directly radiolabeling Ga-68 to αCD. Method: Ga-68 was eluted from the68Ge/68Ga generator. αCD solution was prepared and added to the reaction vial. The vial was heated at 100 °C for 20 min. After that, the reaction was allowed to cool down at room temperature. Radioactivity was measured using instant thin layer chromatography (iTLC) and the obtained iTLC paper was scanned by a TLC scanner. The radiochemical purity (RCP) was calculated. The pH of the resulting solution was measured using a pH meter and its mass to charge ratio was measured using mass spectrometry (MS). The radiosynthesis process was then optimized to increase the RCP of68Ga-αCD. Result: Initially, RCP of the68Ga-αCD was very low (0.236 ± 0.02%, n=3). Upon the optimization using sodium ascorbate as radiostabilizer together with 1 mg/ml αCD solution and purified by C-18 cartridge, the RCP is increased. Ultimately, we have successfully achieved an RCP of 18.4 ± 1.1%. Conclusion: In this study, we successfully radiosynthesized68Ga-αCD by directly radiolabeling Ga-68 with αCD. Although our findings reveal significant possibilities, further investigation of biodistribution studies using PET imaging is required to thoroughly understand the potential of these novel radiopharmaceuticals.