Robust assembly of aldehyde dehydrogenase Ald4p in Saccharomyces cerevisiae
Issued Date
2023-10-01
Resource Type
eISSN
20466390
Scopus ID
2-s2.0-85187952146
Journal Title
Biology Open
Volume
12
Issue
10
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biology Open Vol.12 No.10 (2023)
Suggested Citation
Nasalingkhan C., Sirinonthanawech N., Noree C. Robust assembly of aldehyde dehydrogenase Ald4p in Saccharomyces cerevisiae. Biology Open Vol.12 No.10 (2023). doi:10.1242/bio.060070 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/97738
Title
Robust assembly of aldehyde dehydrogenase Ald4p in Saccharomyces cerevisiae
Author(s)
Author's Affiliation
Corresponding Author(s)
Other Contributor(s)
Abstract
As part of our studies of yeast aldehyde dehydrogenase (Ald4p) assembly, we identified a population of transformants (SWORD strain) that show more robust filament formation of GFP-tagged Ald4p (Ald4p-GFP) than that of a wild type ALD4::GFP strain. Sequencing of the ALD4 gene in the SWORD strain showed that the increased assembly was not due to changes to the ALD4 coding sequence, suggesting that a second mutation site was altering Ald4p assembly. Using short-read whole-genome sequencing, we identified spontaneous mutations in FLO9. Introduction of the SWORD allele of FLO9 into a wild-type ALD4::GFP yeast strain revealed that the changes to FLO9 were a contributor to the increased length of Ald4p- GFP filaments we observe in the SWORD strain and that this effect was not due to an increase in Ald4p protein levels. However, the expression of the FLO9 (SWORD) allele in wild-type yeast did not fully recapitulate the length control defect we observed in SWORD strains, arguing that there are additional genes contributing to the filament length phenotype. For our future work, this FLO9 fromSWORD will be tested whether it could show global effect, promoting the assembly of some other filament-forming enzymes.