Characterization, expression and function of the pyrokinins (PKs) in the giant freshwater prawn, Macrobrachium rosenbergii
Issued Date
2022-06-01
Resource Type
ISSN
00220949
eISSN
14779145
Scopus ID
2-s2.0-85131771886
Pubmed ID
35578905
Journal Title
Journal of Experimental Biology
Volume
225
Issue
11
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Experimental Biology Vol.225 No.11 (2022)
Suggested Citation
Thongbuakaew T., Mukem S., Chaiyamoon A., Khornchatri K., Kruangkum T., Cummins S.F., Sobhon P. Characterization, expression and function of the pyrokinins (PKs) in the giant freshwater prawn, Macrobrachium rosenbergii. Journal of Experimental Biology Vol.225 No.11 (2022). doi:10.1242/jeb.243742 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/83218
Title
Characterization, expression and function of the pyrokinins (PKs) in the giant freshwater prawn, Macrobrachium rosenbergii
Other Contributor(s)
Abstract
Pyrokinins (PKs) are neuropeptides that have been found to regulate a variety of physiological activities including reproduction in various insect and crustacean species. However, the reproductive roles of PKs in the giant freshwater prawn, Macrobrachium rosenbergii, have not yet been investigated. In this study, we identified the MroPK gene from next-generation sequence resources, which encodes a MroPK precursor that shares a high degree of conservation with the C-terminal sequence of FxPRLamide in other arthropods. MroPK is expressed within most tissues, except the hepatopancreas, stomach and gill. Within developing ovarian tissue, MroPK expression was found to be significantly higher during the early stages (stages 1-2) compared with the late stages (stages 3-4), and could be localized to the oogonia, previtellogenic and early vitellogenic oocytes. A role for PK in M. rosenbergii reproduction was supported following experimental administration of MroPK to ovarian explant cultures, which led to an increase in the production of progesterone and estradiol and upregulation of expression of steroidogenesis-related genes (3β-HSD and 17β-HSD) and vitellogenin (Vg). Together, these results support a role for MroPK in regulating ovarian maturation via steroidogenesis.