The susceptibility of some mosquitoes to Wuchereria bancrofti
Issued Date
1991
Copyright Date
1991
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
xxi, 110 leaves : ill. (some col.)
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Mahidol University
Bibliographic Citation
Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1991
Suggested Citation
Jinrapa Phothikasikorn The susceptibility of some mosquitoes to Wuchereria bancrofti. Thesis (M.Sc. (Environmental Biology))--Mahidol University, 1991. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/103283
Title
The susceptibility of some mosquitoes to Wuchereria bancrofti
Alternative Title(s)
การทดสอบความสามารถในการนำเชื้อพยาธิโรคเท้าช้างชนิด Wuchereria bancrofti ของยุงบางชนิด
Author(s)
Abstract
1. Membrane selection for artificial feeding technique Membrane selection for an artificial feeding technique experiment was performed by feeding Aedes aegypti Liverpool strain on the blood of patients infected with Wuchereria bancrofti microfilariae using three types of membranes namely mouse skin chicken skin and layer of seine intestine. Direct feeding on patients by Ae. aegypti was used as a control. The data obtained concerning the survival rate of mosquitoes the rates of infection of osquitoes and the numbers of the third infective stage larvae per mosquito did not differ significantly from those of the control (p>0.05). However the results obtained concerning the response of mosquitoes to feeding of blood through different types of membraned differed significantly; 75.3% 70.0% 32.0% and 84.0% for chicken skin mouse skin layer of swine intestine and direct feeding on patients respectively (p<0.05). Therefore it can be concluded from this experiment that chicken skin is the best skin to be used for artificial feeding technique. 2. Techniques for preservation of microfilariae of Wuchereriabancrofti. Five different types of anti-coagulants namely heparin ACD ACD-DS CPD and CPDA-1 were used to preserve microfilariae of Wuchereria bancrofti obtained from blood of infected patients in order to ascertain as to which one would be the best type of anti-coagulant. Aedes aegypti Liverpool strain was the mosquito selected to feed on preserved blood. The data insecated that microfilariae of W. bancrofti could be preserved in blood by all five anti-coagulant formulae, with CPDA-1 the most and heparin the least satisfactory formulae. The responses of mosquitoes to feeding of patients blood mixed with five anti-coagulant formulae did not differ significantly on each day from the third to the tenth day (P>0.05) Concerning the survival rates of mosquitoes from feeding on patientsblood mixed with five anti-coagulant formulae, the mosquitoes fed on blood mixed with beparin survived until the seventh day whereas those fed on blood miced with the other four anti-coagulant formulae survived throughout the tenth day. Regarding the rates of infection of mosquitoes from feeding on patients blood mixed with five anticoagulant formulae the rates of infection generally decreased as the duration of preservation increased. There were no infections in the mosquitoes fed tith blood mixed with heparin ACD and ACD-D5 on the eighth the tenth and the ninth day respectively whereas those fed with blood mixed with CPD and CPDA-1 infections occurred throughout the tenth day. 3. The susceptibility of some mosquitoes to microfilariae ofWuchereria bancrofti Ten species of mosquitoes namely Aedes aegypti Ae. albopictus Ae. desmotes Ae. niveus comples Anopheles dirus A An. maculatus An. minimus Armigeres subalbatus culex quinquefasciatus and Mansonis uniformis were exposed to microfilariae of the sub-periodic form of Wuchereria bancrofti under laboratory conditions in order to determine their susceptibility to the parasite. The results showed that An. maculatus (73.5%) was the most susceptible to the parasite followed by An. minimus (61.3%) Ae. niveus comples (27.6%) Ae. desmotes (24.8%) An. dirus A (24.5%) Cx. quinquefasciatus (19.4%) Mn. uniformis (9.5%) and Ae. albopictus (0.8%). Ae. aegypti and Ar. subalbatus were not susceptible to the parasite. An. maculatus rendered the highest number of infective larvae 5.7 and Ae. albopictus the least number of infective larvae 1.0
Description
Environmental Biology (Mahidol University 1991)
Degree Name
Master of Science
Degree Level
Master's degree
Degree Department
Faculty of Science
Degree Discipline
Environmental Biology
Degree Grantor(s)
Mahidol University