The Stimulatory Effect of Porcine Placenta Extract on in Vitro Biological Activities of Keratinocytes and Fibroblasts
13
Issued Date
2025-01-01
Resource Type
ISSN
26160684
eISSN
26160692
Scopus ID
2-s2.0-105018868762
Journal Title
Tropical Journal of Natural Product Research
Volume
9
Issue
9
Start Page
4353
End Page
4360
Rights Holder(s)
SCOPUS
Bibliographic Citation
Tropical Journal of Natural Product Research Vol.9 No.9 (2025) , 4353-4360
Suggested Citation
Nensat C., Songjang W., Inpad J., Kijchalao N., Tohtong R., Suthiphongchai T., Pimsen S., Rattanasinganchan P., Metheenukul P., Janvilisri T., Moolthiya P., Sopitthummakhun K., Jiraviriyakul A. The Stimulatory Effect of Porcine Placenta Extract on in Vitro Biological Activities of Keratinocytes and Fibroblasts. Tropical Journal of Natural Product Research Vol.9 No.9 (2025) , 4353-4360. 4360. doi:10.26538/tjnpr/v9i9.34 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/112753
Title
The Stimulatory Effect of Porcine Placenta Extract on in Vitro Biological Activities of Keratinocytes and Fibroblasts
Corresponding Author(s)
Other Contributor(s)
Abstract
Porcine placenta extract (PPE), a synergistic bioactive compound, enhances cellular functions such as proliferation, migration, and cellular signaling. Keratinocytes and fibroblasts play essential roles during wound healing, particularly in tissue regeneration. However, the direct impact of PPE on biological activities of keratinocytes and fibroblasts remains underexplored. This study aimed to elucidate the stimulatory impact of PPE on the proliferation, migration, and gene expression of matrix metalloproteinases (MMPs) and extracellular matrix (ECM)-related proteins in human keratinocytes and fibroblasts. Human keratinocyte (HaCaT) and fibroblast cell lines (Hs 895.Sk) were treated with specific concentrations of PPE (2.5, 5, 10, 12.5, 15, and 50 µg/mL). Cell proliferation and migration were assessed using MTT and scratch assays, respectively. Quantitative real-time PCR was performed to evaluate the expression of MMPs and ECM-related genes. Western blot was employed to investigate the activation of ERK1/2, AKT, JNK signaling pathways, and cyclin D1 expression. PPE significantly promoted the keratinocyte/fibroblast proliferation and migration in a concentration-dependent manner via activation of ERK1/2, AKT, and JNK signaling pathway. PPE also markedly upregulated the expression of MMP-2, MMP-10, and MMP-14 both in keratinocytes and fibroblasts, as well as α-SMA, fibronectin, collagen I, and collagen III in fibroblasts. Porcine placenta extract (PPE) enhances keratinocyte and fibroblast proliferation/migration by activating JNK, ERK1/2, and PI3K/AKT signaling pathways. PPE also upregulates MMP-and ECM-related gene expression including myofibroblast differentiation.