Phytochemical and biological activities of extracts from Impatiens sirindhorniae Triboun & Suksathan

Abstract

Impatiens sirindhorniae Triboun & Suksathan is a succulent plant with pink-purple flowers in the family Balsaminaceae. It is a new endemic species to limestone mountains in Krabi and Suratthani provinces. It has many bioactive compounds. The purpose of this study was to investigate phytochemical compositions and biological activities in leaves of natural I. sirindhorniae, tissue cultured I. sirindhorniae, and I. balsamina L. extracted by ethanol and water. The results showed that the extracts of natural I. sirindhorniae presented higher numbers of phytoconstituents than tissue cultured I. sirindhorniae and I. balsamina L. Ethanol extracts of natural habitat I. sirindhorniae have the highest amount of total phenolic contents (752.94±9.56 mg GAE g-1 extract) and total flavonoid contents (1468.84±50.58 mg Qu g-1 extract). Furthermore, the ethanolic extracts of natural I. sirindhorniae showed the strongest antioxidant activity with IC50 value 6.49 µg mL-1; tyrosinase inhibitory activity with IC50 value 12.01 µg mL-1 and xanthine oxidase inhibitory activity with IC50 value 24.16 µg mL-1. The natural I. sirindhorniae and tissue cultured I. sirindhorniae extracts presented higher biological activities than I. balsamina L. This study revealed that I. sirindhorniae may be a source of natural antioxidant, anti-tyrosinase, and anti-xanthine oxidase compounds which can be used in pharmaceutical and cosmetic formulations following further investigation.