Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP
Issued Date
2023-01-01
Resource Type
ISSN
16879686
eISSN
16879694
Scopus ID
2-s2.0-85168833829
Journal Title
Journal of Tropical Medicine
Volume
2023
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Tropical Medicine Vol.2023 (2023)
Suggested Citation
Kritsiriwuthinan K., Ngrenngarmlert W., Patrapuvich R., Phuagthong S., Choosang K. Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP. Journal of Tropical Medicine Vol.2023 (2023). doi:10.1155/2023/8855171 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/89381
Title
Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP
Author's Affiliation
Other Contributor(s)
Abstract
Considering the importance of merozoite surface proteins (MSPs) as vaccine candidates, this study was conducted to investigate the polymorphism and genetic diversity of Plasmodium vivax merozoite surface protein 3-alpha (PvMSP-3α) in Tailand. To analyze genetic diversity, 118 blood samples containing P. vivax were collected from four malaria-endemic areas in western and southern Tailand. The DNA was extracted and amplifed for the PvMSP-3α gene using nested PCR. The PCR products were genotyped by PCR-RFLP with Hha I and Alu I restriction enzymes. The combination patterns of Hha I and Alu I RFLP were used to identify allelic variants. Genetic evaluation and phylogenic analysis were performed on 13 sequences, including 10 sequences from our study and 3 sequences from GenBank. The results revealed three major types of PvMSP-3α, 91.5% allelic type A (∼1.8 kb), 5.1% allelic type B (∼1.5 kb), and 3.4% allelic type C (∼1.2 kb), were detected based on PCR product size with diferent frequencies. Among all PvMSP-3α, 19 allelic subtypes with Hha I RFLP patterns were distinguished and 6 allelic subtypes with Alu I RFLP patterns were identifed. Of these samples, 73 (61%) and 42 (35.6%) samples were defned as monoallelic subtype infection by Hha I and Alu I PCR-RFLP, respectively, whereas 77 (65.3%) samples were determined to be mixed-allelic subtype infection by the combination patterns of Hha I and Alu I RFLP. These results strongly indicate that PvMSP-3α gene is highly polymorphic, particularly in blood samples collected from the Tai-Myanmar border area (the western part of Tailand). The combination patterns of Hha I and Alu I RFLP of the PvMSP-3α gene could be considered for use as molecular epidemiologic markers for genotyping P. vivax isolates in Tailand.